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首页> 外文期刊>Applied Microbiology >Quantitative PCR Assay for Mycobacterium pseudoshottsii and Mycobacterium shottsii and Application to Environmental Samples and Fishes from the Chesapeake Bay
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Quantitative PCR Assay for Mycobacterium pseudoshottsii and Mycobacterium shottsii and Application to Environmental Samples and Fishes from the Chesapeake Bay

机译：假分支杆菌和分支杆菌的定量PCR分析及其在切萨皮克湾环境样品和鱼类中的应用

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摘要

Striped bass ( Morone saxatilis ) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden ( Brevoortia tyrannus ) and bay anchovy ( Anchoa mitchilli ). Mycobacterium pseudoshottsii was found to be ubiquitous in water samples from the main stem of the Chesapeake Bay and was also present in water and sediments from the Rappahannock River, Virginia. M. pseudoshottsii was also detected in menhaden and anchovy tissues. In contrast, M. shottsii was not detected in water, sediment, or prey fish tissues. In conjunction with its nonpigmented phenotype, which is frequently found in obligately pathogenic mycobacteria of humans, this pattern of occurrence suggests that M. shottsii may be an obligate pathogen of striped bass.
机译：切萨皮克湾的鲈鱼鲈（Morone saxatilis）目前正经历与新描述的分枝杆菌种，假分支杆菌和Shottsii分支杆菌相关的分支杆菌病高发。目前尚不清楚条纹鲈鱼宿主外这些分枝杆菌的生态。在这项工作中，我们开发了对拟shot分枝杆菌和shot分枝杆菌的定量实时PCR分析，并将这些分析应用于切萨皮克湾水和沉积物样品的DNA提取物，以及来自条纹鲈的两个主要猎物大西洋猛禽的组织（Brevoortia tyrannus）和海湾an鱼（Anchoa mitchilli）。在切萨皮克湾主干的水样中发现伪分支杆菌无处不在，并且在弗吉尼亚州拉帕汉诺克河的水和沉积物中也存在。在男子和。鱼组织中也检出了假支原体。相反，在水，沉积物或猎物的鱼组织中未检测到M. Shottsii。结合其在人类专性致病性分枝杆菌中经常发现的非色素表型，这种发生模式表明M. Shottsii可能是条带鲈的专性病原体。

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