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Production of Aflatoxins in Submerged Culture

机译:沉水培养中黄曲霉毒素的生产

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Aflatoxins can be produced on a synthetic medium in submerged culture. Glucose, sucrose, or fructose are the preferred carbon sources, and Casamino Acids are the preferred nitrogen source. Ammonia is almost as good a nitrogen source. Zinc is required at levels of at least 0.4 mg per liter. Concentrations of aflatoxin of 60 to 80 mg per liter (as determined by optical-density measurements of a chloroform extract of the unfiltered broth) can readily be obtained in indented shake flasks; somewhat lower yields were obtained in 5-liter fermentors.
机译:黄曲霉毒素可以在淹没培养的合成培养基上生产。葡萄糖,蔗糖或果糖是优选的碳源,而酪蛋白氨基酸是优选的氮源。氨几乎和氮气一样好。锌的需求量至少为每升0.4毫克。黄曲霉毒素的浓度为60-80 mg / L(由未过滤的肉汤的氯仿提取物的​​光密度测量确定)可以在带有凹口的摇瓶中获得; 5升发酵罐的产量略低。

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