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Elimination of Toxicity from Polyvinyl Trays After Sterilization with Ethylene Oxide

机译：用环氧乙烷灭菌后消除聚乙烯托盘中的毒性

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Ethylene oxide (ETO) sterilization of polyvinyl trays used in an antiviral screening program was initiated to overcome seasonal outbreaks of bacterial and mycotic contamination of tissue culture cells. Trays sterilized by 100% ETO for 5 hr after 48 hr of prehumidification, or by 12% ETO plus 88% Freon 12 for 1, 3, 5 or 18 hr, followed by various methods of aeration, were seeded with several types of tissue culture cells and examined for contamination, toxicity, and monolayer quality. A 1-hr exposure in 12% ETO plus 88% Freon 12 was adequate for sterilization, although residual toxicity for tissue cultures remained. A 7-day aeration period at 37 C was sufficient to eliminate toxicity and allow the growth of good monolayers of WI-38, HEp-2 and primary bovine kidney cells. Sterilization with 100% ETO required 14 days of aeration at 37 C to eliminate cytotoxicity. Increased residual toxicity resulting from longer ETO sterilization periods required longer aeration times at 37 C or higher aeration temperatures for detoxification.
机译：开始了抗病毒筛选程序中使用的聚乙烯托盘的环氧乙烷（ETO）灭菌，以克服组织培养细胞中细菌和霉菌污染的季节性爆发。在预加湿48小时后，将托盘用100％ETO灭菌5 hr，或将12％ETO加88％Freon 12灭菌1、3、5或18 hr，然后通过多种曝气方法对托盘进行灭菌，并接种几种类型的组织培养细胞并检查其污染，毒性和单层质量。尽管保留了组织培养物的残留毒性，但在12％ETO和88％Freon 12中暴露1小时仍足以灭菌。在37°C下进行7天的曝气足以消除毒性并允许WI-38，HEp-2和原代牛肾细胞的良好单层生长。用100％ETO灭菌需要在37°C下通气14天，以消除细胞毒性。较长的ETO灭菌时间导致残留毒性增加，因此在37℃或更高的曝气温度下需要更长的曝气时间以进行排毒。

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