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Cometabolism and Gas Chromatography for the Sensitive Detection of Bacteria

机译：代谢和气相色谱法用于细菌的灵敏检测

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Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus were grown in media containing one of several halogenated organic acids which the bacteria metabolized but could not use as a major carbon and energy source for growth. Gas chromatographic techniques were employed to detect the presence of low population densities by analysis for products formed by the microorganisms. By virtue of the presence of the cometabolizable halogenated substrate in the medium, the sensitivity of procedures for detecting the presence of S. typhimurium, E. coli, and S. aureus was increased more than 7,000-, 20,000-, and 1,000-fold, respectively.
机译：鼠伤寒沙门氏菌，大肠杆菌和金黄色葡萄球菌在含有几种卤代有机酸之一的培养基中生长，细菌可将这些有机酸代谢，但不能用作生长的主要碳和能源。通过分析由微生物形成的产物，采用气相色谱技术检测低种群密度的存在。由于培养基中存在可代谢的卤化底物，因此检测鼠伤寒沙门氏菌，大肠杆菌和金黄色葡萄球菌的方法的灵敏度提高了7,000倍，20,000倍和1,000倍，分别。

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