• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Immunogenicity of Nanogram to Milligram Quantities of Inactivated Foot-and-Mouth Disease Virus. I. Relative Virus-neutralizing Potency of Guinea Pig Sera
【24h】

Immunogenicity of Nanogram to Milligram Quantities of Inactivated Foot-and-Mouth Disease Virus. I. Relative Virus-neutralizing Potency of Guinea Pig Sera

机译：纳米级疫苗对灭活的口蹄疫病毒毫克数量的免疫原性。 I.豚鼠血清的病毒中和能力

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Quantitative antigen dose-neutralizing antibody response curves were established in guinea pigs for purified foot-and-mouth disease virus (FMDV), type A, strain 119, inactivated for 48 hr with N-acetylethyleneimine (AEI). Inactivation of FMDV by 0.05% AEI at 25 C occurred without virus degradation and followed first-order kinetics over a 108-fold decrease in plaque-forming units (PFU) extrapolating to 10-5 PFU/ml at 48 hr. The AEI-treated virus was administered in doses ranging from 10 ng to 2.62 mg, alone or emulsified in oil adjuvant. Sigmoidal dose-response curves were obtained with 160 ng as the minimum effective dose. The maximum effective dose was 163 μg and 2.62 mg or more at 6 and 28 through 84 days postinoculation, respectively. Oil adjuvant had little effect at 6 days postinoculation, but its use markedly increased the amount of neutralizing antibody obtained at the later testing periods.
机译：在豚鼠中建立了纯化的口蹄疫病毒（FMDV）A型，菌株119的定量抗原剂量中和抗体反应曲线，并用N-乙酰基乙亚胺（AEI）灭活了48小时。在25°C时，FMDV被0.05％AEI灭活，没有病毒降解，并且在48小时时，斑块形成单位（PFU）下降了108倍，其一级动力学随即外推至10-5 PFU / ml。单独或在油佐剂中乳化的AEI处理病毒的剂量范围为10 ng至2.62 mg。以160 ng作为最小有效剂量获得了S形剂量反应曲线。接种后第6天和第28天到84天的最大有效剂量分别为163μg和2.62 mg或更高。油佐剂在接种后6天几乎没有作用，但其使用显着增加了在随后的测试期间获得的中和抗体的量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号