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Further Studies on the Anti-Immunoglobulin G Hemagglutination-Inhibition Test for Influenza

机译:抗流感的免疫球蛋白G血凝抑制试验的进一步研究

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Incorporating species-specific anti-immunoglobulin G (IgG) serum into the hemagglutination-inhibition (HI) test for influenza markedly increased sensitivity without loss of specificity. The effectiveness of anti-IgG serum for augmenting antibody titers may be influenced by the variable potency of commercial anti-IgG preparations. Maximal enhancement of HI titers was achieved when anti-IgG serum and virus-antiserum mixtures were incubated at 23 C for 10 min. Precision and reproducibility of the test were within acceptable limits. Other conditions likely to affect the test were investigated.
机译:将物种特异性抗免疫球蛋白G(IgG)血清掺入流感的血凝抑制(HI)测试中可显着提高敏感性,而不会丧失特异性。抗IgG血清增加抗体效价的有效性可能受到市售抗IgG制剂可变效力的影响。当将抗IgG血清和病毒-抗血清混合物在23 C孵育10分钟时,HI效价最大程度提高。测试的精度和可重复性均在可接受的范围内。研究了可能影响测试的其他条件。

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