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Simplified Gas Chromatographic Procedure for Identification of Bacterial Metabolic Products

机译:简化的气相色谱法鉴定细菌代谢产物

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A rapid and simple procedure is described for analysis of fermentation products from anaerobic bacteria grown in glucose broth. A 1-ml sample of the culture is drained through cation-exchange resin in a Pasteur pipette. The effluent fluid is directly analyzed isothermally in a gas chromatograph for volatile fatty acids (C2 to C6) as well as for lactic, pyruvic, and succinic acids. This procedure is considered to be suitable for routine use in clinical bacteriology.
机译:描述了一种快速简单的程序,用于分析葡萄糖肉汤中生长的厌氧细菌的发酵产物。通过巴斯德移液管中的阳离子交换树脂将1 ml培养物样品排干。直接在气相色谱仪中等温分析废水中的挥发性脂肪酸(C2至C6)以及乳酸,丙酮酸和琥珀酸。该程序被认为适合常规用于临床细菌学。

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