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Occurrence of a Complex Form of Staphylokinase in the Course of Cultivation of Staphylococcus aureus

机译:金黄色葡萄球菌培养过程中葡萄球激酶复合形式的发生

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Proteins in the culture supernatant of Staphylococcus aureus PS 47 were subjected to Sephadex chromatography. In the early stage of the cultivation, staphylokinase appeared to have a molecular weight of 15,000 and in the later stage it appeared to have a molecular weight of 320,000. The staphylokinase having a lower molecular weight (type A) converted into one having a higher value (type B) during the course of cultivation. It was demonstrated that conversion of type A into type B took place in vitro (monitored by gel filtration after the two types of staphylokinases were mixed), and it was observed that type B reverted to type A when type B was treated with KCl or detergent. Type B seems to be a complex form of type A and some high-molecular-weight substance.
机译:对金黄色葡萄球菌PS 47的培养上清液中的蛋白质进行Sephadex层析。在培养的早期,葡萄激酶的分子量似乎为15,000,而在后期则似乎为320,000。在培养过程中,具有较低分子量(A型)的葡萄激酶被转化为具有较高值(B型)的葡萄激酶。证明了A型向B型的转化是在体外发生的(两种葡萄球菌酶混合后通过凝胶过滤监测),并且观察到当用KCl或去污剂处理B型时B型恢复为A型。 。 B型似乎是A型和某些高分子量物质的复杂形式。

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