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首页> 外文期刊>Applied Microbiology >Carboxylate Transporter Gene JEN1 from the Entomopathogenic Fungus Beauveria bassiana Is Involved in Conidiation and Virulence
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Carboxylate Transporter Gene JEN1 from the Entomopathogenic Fungus Beauveria bassiana Is Involved in Conidiation and Virulence

机译：来自病原性真菌球孢白僵菌的羧酸盐转运蛋白基因JEN1参与分生和毒力。

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摘要

Beauveria bassiana is an important entomopathogenic fungus widely used as a biological agent to control insect pests. A gene ( B. bassiana JEN1 [ BbJEN1 ]) homologous to JEN1 encoding a carboxylate transporter in Saccharomyces cerevisiae was identified in a B. bassiana transfer DNA (T-DNA) insertional mutant. Disruption of the gene decreased the carboxylate contents in hyphae, while increasing the conidial yield. However, overexpression of this transporter resulted in significant increases in carboxylates and decreased the conidial yield. BbJEN1 was strongly induced by insect cuticles and highly expressed in the hyphae penetrating insect cuticles not in hyphal bodies, suggesting that this gene is involved in the early stage of pathogenesis of B. bassiana . The bioassay results indicated that disruption of BbJEN1 significantly reduced the virulence of B. bassiana to aphids. Compared to the wild type, Δ BbJEN1 alkalinized the insect cuticle to a reduced extent. The alkalinization of the cuticle is a physiological signal triggering the production of pathogenicity. Therefore, we identified a new factor influencing virulence, which is responsible for the alkalinization of the insect cuticle and the initiation of fungal pathogenesis in insects.
机译：球孢白僵菌是一种重要的昆虫病原真菌，被广泛用作控制害虫的生物制剂。在啤酒球菌转移DNA（T-DNA）插入突变体中鉴定了与酿酒酵母中编码羧酸盐转运蛋白的JEN1同源的基因（球孢杆菌JEN1 [BbJEN1]）。基因的破坏降低了菌丝中的羧酸盐含量，同时增加了分生孢子的产率。然而，这种转运蛋白的过表达导致羧酸盐的显着增加并降低了分生孢子产率。 BbJEN1受到昆虫表皮的强烈诱导，并在菌丝穿透的昆虫表皮中而不在菌丝体中高表达，这表明该基因参与了球孢杆菌病的早期发病。生物测定结果表明，BbJEN1的破坏显着降低了球孢白僵菌对蚜虫的毒力。与野生型相比，ΔBbJEN1将昆虫表皮碱化的程度有所降低。角质层的碱化是触发致病性产生的生理信号。因此，我们确定了一个影响毒力的新因素，该因素负责昆虫表皮的碱化和昆虫中真菌发病机理的启动。

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