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首页> 外文期刊>Applied Microbiology >Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet
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Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet

机译：过渡到高粮饮食期间牛肉牛瘤胃中细菌多样性与上皮组织相关的变化

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Our understanding of the ruminal epithelial tissue-associated bacterial (defined as epimural bacteria in this study) community is limited. In this study, we aimed to determine whether diet influences the diversity of the epimural bacterial community in the bovine rumen. Twenty-four beef heifers were randomly assigned to either a rapid grain adaptation (RGA) treatment ( n = 18) in which the heifers were allowed to adapt from a diet containing 97% hay to a diet containing 8% hay over 29 days or to the control group ( n = 6), which was fed 97% hay. Rumen papillae were collected when the heifers were fed 97%, 25%, and 8% hay diets. PCR-denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR analysis were used to characterize rumen epimural bacterial diversity and to estimate the total epimural bacterial population (copy numbers of the 16S rRNA gene). The epimural bacterial diversity from RGA heifers changed ( P = 0.01) in response to the rapid dietary transition, whereas it was not affected in control heifers. A total of 88 PCR-DGGE bands were detected, and 44 were identified from phyla including Firmicutes , Bacteroidetes , and Proteobacteria . The bacteria Treponema sp., Ruminobacter sp., and Lachnospiraceae sp. were detected only when heifers were fed 25% and 8% hay diets, suggesting the presence of these bacteria is the result of adaptation to the high-grain diets. In addition, the total estimated population of rumen epimural bacteria was positively correlated with molar proportions of acetate, isobutyrate, and isovalerate, suggesting that they may play a role in volatile fatty acid metabolism in the rumen.
机译：我们对瘤胃上皮组织相关细菌（在本研究中定义为硬膜细菌）群落的了解有限。在这项研究中，我们旨在确定饮食是否影响牛瘤胃中硬膜外细菌群落的多样性。 24个牛肉小母牛被随机分配到一种快速谷物适应（RGA）处理中（n = 18），在这种情况下，允许小母牛在29天之内从含97％干草的日粮改成含8％干草的日粮，或对照组（n = 6），喂了97％的干草。当给小母牛饲喂97％，25％和8％的干草日粮时，会收集瘤胃乳头。 PCR变性梯度凝胶电泳（DGGE）和定量实时PCR分析用于表征瘤胃硬膜外细菌的多样性，并估计总硬膜外细菌的数量（16S rRNA基因的拷贝数）。 RGA小母牛的硬膜外细菌多样性响应快速的饮食过渡而发生了变化（P = 0.01），而对照小母牛则没有受到影响。共检测到88条PCR-DGGE条带，并从门上鉴定出44条，包括硬毛菌，拟杆菌属和变形杆菌。细菌有梅毒螺旋体，Ruminobacter属和Lachnospiraceae属。仅在饲喂25％和8％的干草饲喂小母牛时才检测到这种细菌，这表明这些细菌的存在是适应高谷物饮食的结果。此外，瘤胃硬膜外细菌的估计总数与乙酸盐，异丁酸盐和异戊酸的摩尔比例呈正相关，表明它们可能在瘤胃中的挥发性脂肪酸代谢中起作用。

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