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首页> 外文期刊>Applied Microbiology >Fecal Bacteroidales Diversity and Decay in Response to Variations in Temperature and Salinity
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Fecal Bacteroidales Diversity and Decay in Response to Variations in Temperature and Salinity

机译：粪细菌细菌的多样性和温度和盐度变化的响应的衰减。

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Bacteroidales are attractive as water quality indicators because of their potential to discern sources of fecal pollution, and it is presumed that these bacteria do not multiply outside their host organisms. The persistence of a fecal Bacteroidales marker was monitored over 14 days in river water microcosms that varied in temperature from 10°C to 30°C and salinity from 0‰ to 30‰ by quantitative PCR (qPCR). Decay rates were estimated and compared to the results of other studies examining the survival and persistence of Bacteroidales markers by converting decay rates from other studies to a common decay rate unit. The log-linear decay rates estimated in this work ranged from ?0.18 to ?1.31 ln( C _( T )/ C _(0)) day~(?1), where C _( T ) is the threshold cycle and C _(0) is the concentration of cells at time zero, which is comparable to findings in previous studies. Salinity had a positive effect on Bacteroidales marker persistence, while decay was more rapid at higher temperatures. Comparison of 16S rRNA gene clone libraries generated from microcosm samples indicated that most of the operational taxonomic unit (OTU) and phylogenetic diversity was found within samples and not between samples, indicating at least qualitatively that diverse lineages persist and likely have similar survival characteristics under most of the conditions examined. It was noted that the samples at higher salinities also had the smallest amount of diversity between samples as well as the lowest decay rates. This research also highlights the need for a repository of raw survival and persistence data if more sophisticated models of decay are to be employed and compared between different studies.
机译：由于细菌具有识别粪便污染源的潜力，因此其作为水质指标具有吸引力，并且推测这些细菌不会在其宿主生物体之外繁殖。通过定量PCR（qPCR）在温度从10°C到30°C和盐度从0‰到30‰变化的河水微观环境中监测粪便细菌标记的持续时间超过14天。通过将衰变速率从其他研究转换为通用的衰变速率单位，可以估算出衰变速率，并将其与检查细菌类标记物存活和持久性的其他研究结果进行比较。在这项工作中，估计的对数线性衰减率范围为？0.18至？1.31 ln（C _（T）/ C _（0））day〜（？1），其中C _（T）是阈值周期，而C _（0）是零时的细胞浓度，与以前的研究结果相当。盐度对拟杆菌的持久性有积极的影响，而在较高的温度下腐烂更快。比较从微观样品产生的16S rRNA基因克隆文库，表明大多数可操作分类单位（OTU）和系统发生多样性均在样品内发现，而不是在样品间发现，至少定性地表明，不同谱系仍然存在，并且在大多数情况下可能具有相似的生存特征检查的条件。要注意的是，较高盐度的样品之间的多样性也最小，衰减率最低。如果要使用更复杂的衰减模型并在不同研究之间进行比较，则本研究还强调需要原始生存和持久性数据的存储库。

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