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首页> 外文期刊>Applied Microbiology >Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom
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Changes in Dimethylsulfoniopropionate Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom

机译：诱导的浮游植物绽放反应中二甲基磺丙酸脱甲基酶基因组装的变化

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Over half of the bacterioplankton cells in ocean surface waters are capable of carrying out a demethylation of the phytoplankton metabolite dimethylsulfoniopropionate (DMSP) that routes the sulfur moiety away from the climatically active gas dimethylsulfide (DMS). In this study, we tracked changes in dmdA , the gene responsible for DMSP demethylation, over the course of an induced phytoplankton bloom in Gulf of Mexico seawater microcosms. Analysis of >91,000 amplicon sequences indicated 578 different dmdA sequence clusters at a conservative clustering criterion of ≥90% nucleotide sequence identity over the 6-day study. The representation of the major clades of dmdA , several of which are linked to specific taxa through genomes of cultured marine bacterioplankton, remained fairly constant. However, the representation of clusters within these major clades shifted significantly in response to the bloom, including two Roseobacter -like clusters and a SAR11-like cluster, and the best correlate with shifts of the dominant dmdA clades was chlorophyll a concentration. Concurrent 16S rRNA amplification and sequencing indicated the presence of Roseobacter , SAR11, OM60, and marine Rhodospirillales populations, all of which are known to harbor dmdA genes, although the largest taxonomic change was an increase in Flavobacteriaceae , a group not yet demonstrated to have DMSP-demethylating capabilities. Sequence heterogeneity in dmdA and other functional gene populations is becoming increasingly evident with the advent of high-throughput sequencing technologies, and understanding the ecological implications of this heterogeneity is a major challenge for marine microbial ecology.
机译：海洋表面水域中超过一半的浮游细菌细胞能够对浮游生物代谢产物二甲基磺丙酸二甲酯（DMSP）进行脱甲基，从而使硫部分远离气候活性气体二甲基硫（DMS）。在这项研究中，我们追踪了在墨西哥湾海水缩影中诱导的浮游植物开花过程中，导致DMSP去甲基化的基因dmdA的变化。在为期6天的研究中，对≥91,000个扩增子序列进行的分析表明，在保守性聚类标准下，≥578％核苷酸序列同一性，可得到578个不同的dmdA序列簇。 dmdA主要进化枝的代表保持相当恒定，其中dmdA的主要进化枝通过培养的海洋浮游生物的基因组与特定的分类群相关联。然而，这些主要进化枝中的簇代表随着花开而发生了显着变化，包括两个类似玫瑰红杆菌的簇和一个SAR11样簇，并且与主要dmdA进化枝的移位最相关的是叶绿素a浓度。同时进行的16S rRNA扩增和测序表明存在Roseobacter，SAR11，OM60和海洋螺旋藻种群，所有这些种群都带有dmdA基因，尽管最大的分类学变化是Flavobacteriaceae的增加，而Flavobacteriaceae尚未被证明具有DMSP。脱甲基能力。随着高通量测序技术的出现，dmdA和其他功能基因种群中的序列异质性变得越来越明显，而了解这种异质性的生态意义是海洋微生物生态学面临的主要挑战。

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