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首页> 外文期刊>Applied Microbiology >Poly-3-Hydroxybutyrate Metabolism in the Type II Methanotroph Methylocystis parvus OBBP
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Poly-3-Hydroxybutyrate Metabolism in the Type II Methanotroph Methylocystis parvus OBBP

机译：II型甲基营养型甲基囊藻小孢子OBBP中的聚-3-羟基丁酸代谢

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Differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (PHB). Previous researchers have demonstrated that PHB functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how C_(1) metabolism affects the role of PHB. In the present study, the type II methanotroph Methylocystis parvus OBBP did not replicate using stored PHB in the absence of methane, even when all other nutrients were provided in excess. When PHB-rich cultures of M. parvus OBBP were deprived of carbon and nitrogen for 48 h, they did not utilize significant amounts of stored PHB, and neither cell concentrations nor concentrations of total suspended solids changed significantly. When methane and nitrogen both were present, PHB and methane were consumed simultaneously. Cells with PHB had significantly higher specific growth rates than cells lacking PHB. The addition of formate (a source of reducing power) to PHB-rich cells delayed PHB consumption, but the addition of glyoxylate (a source of C_(2) units) did not. This and results from other researchers suggest that methanotrophic PHB metabolism is linked to the supply of reducing power as opposed to the supply of C_(2) units for synthesis.
机译：生物之间碳同化途径的差异和降低功率的要求可能会影响存储聚合物聚3-羟基丁酸酯（PHB）的作用。先前的研究人员已经证明，在碳缺乏期，PHB在富含乙酸盐的需氧培养物中充当唯一的生长底物，但尚不确定C_（1）代谢如何影响PHB的作用。在本研究中，即使在没有其他甲烷的情况下，即使没有提供其他所有营养素，也无法使用储存的PHB复制II型甲烷营养型小孢子甲基囊藻OBBP。当富含PHB的小分枝杆菌OBBP的碳和氮被剥夺48小时时，它们并没有利用大量的PHB，细胞浓度和总悬浮固体的浓度也没有明显变化。当甲烷和氮气都存在时，PHB和甲烷同时消耗。具有PHB的细胞比没有PHB的细胞具有明显更高的比生长速率。向富PHB的细胞中添加甲酸（降低功率的来源）可延迟PHB的消耗，但不添加乙醛酸酯（C_（2）单位的来源）。这和其他研究人员得出的结果表明，甲烷营养型PHB代谢与还原能力的供应有关，而不是与C_（2）单元的供应有关。

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