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首页> 外文期刊>Applied Microbiology >Patterns of [FeFe] Hydrogenase Diversity in the Gut Microbial Communities of Lignocellulose-Feeding Higher Termites
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Patterns of [FeFe] Hydrogenase Diversity in the Gut Microbial Communities of Lignocellulose-Feeding Higher Termites

机译：木质纤维素饲喂高白蚁肠道微生物群落中[FeFe]氢化酶多样性的模式。

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Hydrogen is the central free intermediate in the degradation of wood by termite gut microbes and can reach concentrations exceeding those measured for any other biological system. Degenerate primers targeting the largest family of [FeFe] hydrogenases observed in a termite gut metagenome have been used to explore the evolution and representation of these enzymes in termites. Sequences were cloned from the guts of the higher termites Amitermes sp. strain Cost010, Amitermes sp. strain JT2, Gnathamitermes sp. strain JT5, Microcerotermes sp. strain Cost008, Nasutitermes sp. strain Cost003, and Rhyncotermes sp. strain Cost004. Each gut sample harbored a more rich and evenly distributed population of hydrogenase sequences than observed previously in the guts of lower termites and Cryptocercus punctulatus. This accentuates the physiological importance of hydrogen for higher termite gut ecosystems and may reflect an increased metabolic burden, or metabolic opportunity, created by a lack of gut protozoa. The sequences were phylogenetically distinct from previously sequenced [FeFe] hydrogenases. Phylogenetic and UniFrac comparisons revealed congruence between host phylogeny and hydrogenase sequence library clustering patterns. This may reflect the combined influences of the stable intimate relationship of gut microbes with their host and environmental alterations in the gut that have occurred over the course of termite evolution. These results accentuate the physiological importance of hydrogen to termite gut ecosystems.
机译：氢是白蚁肠道微生物降解木材的主要自由中间产物，其浓度可能超过任何其他生物系统所测量的浓度。在白蚁肠道元基因组中观察到的针对最大的[FeFe]氢化酶家族的简并引物已用于探索这些酶在白蚁中的进化和表达。从高级白蚁Amitermes sp。的内脏克隆序列。菌株Cost010，Amitermes sp。菌株JT2，Gnathamitermes sp。菌株JT5，Microcerotermes sp。菌株Cost008，Nasutitermes sp。菌株Cost003和Rhyncotermes sp。菌株Cost004。每个肠道样品中都带有比以前在小白蚁和点刺隐孢子虫的肠道中观察到的更丰富，分布更均匀的氢化酶序列。这突显了氢对于高级白蚁肠道生态系统的生理重要性，并且可能反映了由于缺乏肠道原生动物而增加的代谢负担或代谢机会。该序列在系统发育上不同于先前测序的[FeFe]氢化酶。系统发育和UniFrac比较显示主机系统发育和氢化酶序列库聚类模式之间的一致性。这可能反映了肠道微生物与其宿主之间稳定的亲密关系的综合影响，以及在白蚁进化过程中发生的肠道环境变化的综合影响。这些结果强调了氢对白蚁肠道生态系统的生理重要性。

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