• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans
【24h】

Expression of Chlorite Dismutase and Chlorate Reductase in the Presence of Oxygen and/or Chlorate as the Terminal Electron Acceptor in Ideonella dechloratans

机译：氧和/或氯酸盐作为脱氯小球藻终端电子受体存在时亚氯酸盐歧化酶和氯酸盐还原酶的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ability of microorganisms to perform dissimilatory (per)chlorate reduction is, for most species, known to be oxygen sensitive. Consequently, bioremediation processes for the removal of oxochlorates will be disturbed if oxygen is present. We measured the expression of chlorite dismutase and chlorate reductase in the presence of different terminal electron acceptors in the chlorate reducer Ideonella dechloratans . Enzyme activity assays and mRNA analyses by real-time quantitative reverse transcription (qRT)-PCR were performed on cell extracts from cells grown aerobically with and without chlorate and on cells grown anaerobically in the presence of chlorate. Our results showed that both chlorite dismutase and chlorate reductase are expressed during aerobic growth. However, transfer to anaerobic conditions with chlorate resulted in significantly enhanced enzyme activities and mRNA levels for both enzymes. Absence of oxygen was necessary for the induction to occur, since chlorate addition under aerobic conditions produced neither increased enzyme activities nor higher relative levels of mRNA. For chlorite dismutase, the observed increase in activity was on the same order of magnitude as the increase in the relative mRNA level, indicating gene regulation at the transcriptional level. However, chlorate reductase showed about 200 times higher enzyme activity in anaerobically induced cells, whereas the increase in mRNA was only about 10-fold, suggesting additional mechanisms influence the enzyme activity.
机译：对于大多数物种来说，微生物执行异化（过）氯酸盐还原的能力已知对氧敏感。因此，如果存在氧气，则用于去除草酰氯的生物修复过程将受到干扰。我们测量了在氯酸盐还原剂小麦球藻中不同末端电子受体存在下亚氯酸盐歧化酶和氯酸盐还原酶的表达。通过实时定量逆转录（qRT）-PCR进行酶活性测定和mRNA分析，对有氧和无氯条件下需氧生长的细胞的细胞提取物，以及在有氯酸盐的情况下厌氧生长的细胞进行的酶提取。我们的结果表明，有氧生长过程中都表达了亚氯酸盐歧化酶和氯酸盐还原酶。但是，用氯酸盐转移到厌氧条件下会显着提高两种酶的酶活性和mRNA水平。氧的诱导是必需的，因为在有氧条件下加入氯酸盐既不会增加酶的活性，也不会产生更高的mRNA相对水平。对于亚氯酸盐歧化酶，观察到的活性增加与相对mRNA水平的增加在相同数量级上，表明基因在转录水平上调控。然而，氯酸还原酶在厌氧诱导的细胞中显示出约200倍的酶活性，而mRNA的增加仅约10倍，表明其他机制可影响酶的活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号