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首页> 外文期刊>Applied Microbiology >High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days
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High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days

机译：高通量序列分析表达苏云金芽孢杆菌Cry1Ab（Bt玉米）的转基因饲喂MON810玉米的断奶仔猪肠道菌群31天

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The objective of this study was to investigate if feeding genetically modified (GM) MON810 maize expressing the Bacillus thuringiensis insecticidal protein (Bt maize) had any effects on the porcine intestinal microbiota. Eighteen pigs were weaned at ～28 days and, following a 6-day acclimatization period, were assigned to diets containing either GM (Bt MON810) maize or non-GM isogenic parent line maize for 31 days ( n = 9/treatment). Effects on the porcine intestinal microbiota were assessed through culture-dependent and -independent approaches. Fecal, cecal, and ileal counts of total anaerobes, Enterobacteriaceae , and Lactobacillus were not significantly different between pigs fed the isogenic or Bt maize-based diets. Furthermore, high-throughput 16S rRNA gene sequencing revealed few differences in the compositions of the cecal microbiotas. The only differences were that pigs fed the Bt maize diet had higher cecal abundance of Enterococcaceae (0.06 versus 0%; P < 0.05), Erysipelotrichaceae (1.28 versus 1.17%; P < 0.05), and Bifidobacterium (0.04 versus 0%; P < 0.05) and lower abundance of Blautia (0.23 versus 0.40%; P < 0.05) than pigs fed the isogenic maize diet. A lower enzyme-resistant starch content in the Bt maize, which is most likely a result of normal variation and not due to the genetic modification, may account for some of the differences observed within the cecal microbiotas. These results indicate that Bt maize is well tolerated by the porcine intestinal microbiota and provide additional data for safety assessment of Bt maize. Furthermore, these data can potentially be extrapolated to humans, considering the suitability of pigs as a human model.
机译：这项研究的目的是调查表达苏云金芽孢杆菌杀虫蛋白（Bt玉米）的转基因（GM）MON810玉米对猪肠道菌群是否有任何影响。在〜28天时对18头猪断奶，经过6天的适应期后，将其分配给含有GM（Bt MON810）玉米或非GM等基因亲本玉米的日粮31天（n = 9 /次）。通过培养依赖性和非依赖性方法评估了对猪肠道菌群的影响。饲喂等基因或基于Bt玉米的日粮的猪中，总厌氧菌，肠杆菌科和乳杆菌的粪便，盲肠和回肠计数没有显着差异。此外，高通量16S rRNA基因测序显示盲肠菌群的组成几乎没有差异。唯一的区别是，饲喂Bt玉米日粮的猪的肠球菌盲肠丰度较高（0.06比0％; P <0.05），红藻科（1.28比1.17％; P <0.05）和双歧杆菌（0.04比0％; P < 0.05）和比饲喂同基因玉米日粮的猪低的蓝藻丰度（0.23比0.40％; P <0.05）。 Bt玉米中较低的酶抗性淀粉含量很可能是正常变异的结果，而不是由于基因改造所致，可能是盲肠菌群中观察到的一些差异。这些结果表明，Bt玉米对猪肠道菌群的耐受性良好，并为Bt玉米的安全性评估提供了其他数据。此外，考虑到猪是否适合作为人类模型，这些数据可能会外推给人类。

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