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Microbial Functional Gene Diversity with a Shift of Subsurface Redox Conditions during In Situ Uranium Reduction

机译：微生物功能基因多样性与原位还原铀过程中地下氧化还原条件的变化

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To better understand the microbial functional diversity changes with subsurface redox conditions during in situ uranium bioremediation, key functional genes were studied with GeoChip, a comprehensive functional gene microarray, in field experiments at a uranium mill tailings remedial action (UMTRA) site (Rifle, CO). The results indicated that functional microbial communities altered with a shift in the dominant metabolic process, as documented by hierarchical cluster and ordination analyses of all detected functional genes. The abundance of dsrAB genes (dissimilatory sulfite reductase genes) and methane generation-related mcr genes (methyl coenzyme M reductase coding genes) increased when redox conditions shifted from Fe-reducing to sulfate-reducing conditions. The cytochrome genes detected were primarily from Geobacter sp. and decreased with lower subsurface redox conditions. Statistical analysis of environmental parameters and functional genes indicated that acetate, U(VI), and redox potential (E_(h)) were the most significant geochemical variables linked to microbial functional gene structures, and changes in microbial functional diversity were strongly related to the dominant terminal electron-accepting process following acetate addition. The study indicates that the microbial functional genes clearly reflect the in situ redox conditions and the dominant microbial processes, which in turn influence uranium bioreduction. Microbial functional genes thus could be very useful for tracking microbial community structure and dynamics during bioremediation.
机译：为了更好地了解在原位铀生物修复过程中微生物功能多样性随地下氧化还原条件的变化，在铀厂尾矿修复作用（UMTRA）现场（Rifle，CO）的现场实验中，使用综合功能基因芯片GeoChip研究了关键功能基因）。结果表明，功能性微生物群落随着主要代谢过程的改变而改变，如通过对所有检测到的功能性基因的层次聚类和排序分析所证明的。当氧化还原条件从铁还原条件转变为硫酸还原条件时，dsrAB基因（异化亚硫酸还原酶基因）和甲烷生成相关的mcr基因（甲基辅酶M还原酶编码基因）的丰度增加。检测到的细胞色素基因主要来自Geobacter sp。并随着较低的地下氧化还原条件而降低。对环境参数和功能基因的统计分析表明，乙酸盐，U（VI）和氧化还原电势（E_（h））是与微生物功能基因结构相关的最重要的地球化学变量，微生物功能多样性的变化与微生物的功能密切相关。加入乙酸盐后，主要的末端电子接受过程。研究表明，微生物功能基因清楚地反映了原位氧化还原条件和主要的微生物过程，从而影响了铀的生物还原。因此，微生物功能基因对于在生物修复过程中追踪微生物群落结构和动力学可能非常有用。

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