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首页> 外文期刊>Applied Microbiology >Identification and Regulation of fusA, the Polyketide Synthase Gene Responsible for Fusarin Production in Fusarium fujikuroi
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Identification and Regulation of fusA, the Polyketide Synthase Gene Responsible for Fusarin Production in Fusarium fujikuroi

机译：fusA的鉴定和调控，fusA，负责富士镰刀菌镰刀菌生产的聚酮化合物合酶基因

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摘要

Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin-producing fungus Fusarium fujikuroi . Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA . The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen availability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduction of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cellophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum . The fusA mutants, however, are not affected in their capacities to grow on plant tissues.
机译：镰刀菌素是由不同的镰刀菌属物种产生的一类聚酮化合物真菌毒素，包括产生赤霉素的真菌镰刀菌镰刀菌。基于在其他镰刀菌菌株中生产富沙林的聚酮化合物合酶（PKS）酶之间的序列比较，我们确定了F.fujikuroi直系同源物，称为fusA。通过有针对性的诱变证明了fusA参与fusArin生物合成。真菌中的氮可利用性短暂地刺激镰孢菌素的产生，这种调节与细胞中的fusA mRNA水平平行。培养物的照明导致fusalin含量的降低，这种作用在一定程度上可以解释为这些化合物对光的高度敏感性。除了缺乏fusarins的特征性黄色和/或橙色色素沉着之外，fusA基因的突变体没有表现出外部表型改变，包括形态和分生孢子。此外，fusA突变体在降解琼脂培养物上的玻璃纸方面不如野生型有效，这是与尖孢镰刀菌的发病机理有关的特征。然而，fusA突变体在植物组织上生长的能力没有受到影响。

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