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首页> 外文期刊>Applied Microbiology >Molecular Characterization of Commensal Escherichia coli Adapted to Different Compartments of the Porcine Gastrointestinal Tract
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Molecular Characterization of Commensal Escherichia coli Adapted to Different Compartments of the Porcine Gastrointestinal Tract

机译：适应猪胃肠道不同区室的大肠埃希菌的分子特征

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The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms ( n = 146) isolated from different regions of the intestinal tracts of eight pigs (dueodenum, ileum, colon, and feces). The isolates were typed using the method of random amplified polymorphic DNA (RAPD) and screened for the presence of bacteriocin genes and plasmid replicon types. Molecular analysis of variance using the RAPD data showed that E. coli isolates are nonrandomly distributed among different gut regions, and that gut region accounted for 25% ( P < 0.001) of the observed variation among strains. Bacteriocin screening revealed that a bacteriocin gene was detected in 45% of the isolates, with 43% carrying colicin genes and 3% carrying microcin genes. Of the bacteriocins observed (H47, E3, E1, E2, E7, Ia/Ib, and B/M), the frequency with which they were detected varied with respect to gut region for the colicins E2, E7, Ia/Ib, and B/M. The plasmid replicon typing gave rise to 25 profiles from the 13 Inc types detected. Inc F types were detected most frequently, followed by Inc HI1 and N types. Of the Inc types detected, 7 were nonrandomly distributed among isolates from the different regions of the gut. The results of this study indicate that not only may the different regions of the gastrointestinal tract harbor different strains of E. coli but also that strains from different regions have different characteristics.
机译：大肠杆菌作为病原体的作用一直是大量研究的重点，而关于大肠杆菌的表述以及它如何适应和定居哺乳动物肠道内的不同环境方面则知之甚少。在这项研究中，我们表征了从八头猪（十二指肠，回肠，结肠和粪便）的肠道不同区域分离的大肠杆菌（n = 146）。使用随机扩增多态性DNA（RAPD）方法对分离株进行分型，并筛选细菌素基因和质粒复制子类型的存在。使用RAPD数据对变异进行的分子分析表明，大肠杆菌分离株在不同肠道区域之间非随机分布，并且肠道区域占菌株间观察到的变异的25％（P <0.001）。细菌素筛选显示，在45％的分离物中检测到细菌素基因，其中43％携带大肠菌素基因和3％携带微素基因。在观察到的细菌素中（H47，E3，E1，E2，E7，Ia / Ib和B / M），其检出频率相对于大肠菌素E2，E7，Ia / Ib和B7的肠道区域而有所不同。 B / M。质粒复制子分型从所检测的13种Inc类型产生了25个图谱。最常见的是Inc F类型，其次是Inc HI1和N类型。在检测到的公司类型中，有7种非随机分布在来自肠道不同区域的分离株中。这项研究的结果表明，不仅胃肠道的不同区域可能带有不同的大肠杆菌菌株，而且不同地区的菌株也具有不同的特性。

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