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首页> 外文期刊>Applied Microbiology >Effects of UV-B Radiation and Periodic Desiccation on the Morphogenesis of the Edible Terrestrial Cyanobacterium Nostoc flagelliforme
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Effects of UV-B Radiation and Periodic Desiccation on the Morphogenesis of the Edible Terrestrial Cyanobacterium Nostoc flagelliforme

机译：UV-B辐射和周期性干燥对可食陆地蓝藻成虫鞭毛形态发生的影响

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The terrestrial cyanobacterium Nostoc flagelliforme Berk. et M. A. Curtis has been a popular food and herbal ingredient for hundreds of years. To meet great market demand and protect the local ecosystem, for decades researchers have tried to cultivate N. flagelliforme but have failed to get macroscopic filamentous thalli. In this study, single trichomes with 50 to 200 vegetative cells were induced from free-living cells by low light and used to investigate the morphogenesis of N. flagelliforme under low UV-B radiation and periodic desiccation. Low-fluence-rate UV-B (0.1 W m~(?2)) did not inhibit trichome growth; however, it significantly increased the synthesis of extracellular polysaccharides and mycosporine-like amino acids and promoted sheath formation outside the trichomes. Under low UV-B radiation, single trichomes developed into filamentous thalli more than 1 cm long after 28 days of cultivation, most of which grew separately in liquid BG11 medium. With periodic desiccation treatment, the single trichomes formed flat or banded thalli that grew up to 2 cm long after 3 months on solid BG11 medium. When trichomes were cultivated on solid BG11 medium with alternate treatments of low UV-B and periodic desiccation, dark and scraggly filamentous thalli that grew up to about 3 cm in length after 40 days were obtained. In addition, the cultivation of trichomes on nitrogen-deficient solid BG11 medium (BG11_(0)) suggested that nitrogen availability could affect the color and lubricity of newly developed thalli. This study provides promising techniques for artificial cultivation of N. flagelliforme in the future.
机译：陆地蓝细菌Nostoc flagelliforme Berk。 et M. A. Curtis数百年来一直是一种流行的食品和草药成分。为了满足巨大的市场需求并保护当地的生态系统，数十年来，研究人员一直在尝试种植鞭毛猪笼草，但未能获得肉眼可见的丝状拟南芥。在这项研究中，通过弱光从自由生活的细胞中诱导出具有50至200个营养细胞的单个毛状体，并用于研究在低UV-B辐射和周期性干燥下鞭毛猪笼草的形态发生。低通量率的UV-B（0.1 W m〜（？2））不能抑制毛状体的生长。然而，它显着增加了细胞外多糖和霉菌素样氨基酸的合成，并促进了毛状体外鞘的形成。在低UV-B辐射下，经过28天的培养，单个毛状体长成了1厘米长的丝状藻，其中大部分在液体BG11培养基中单独生长。经过定期的干燥处理，单个的毛状体在扁平的BG11培养基上形成3个月后长到2厘米长的扁平或带状的拟南芥。当将毛状体在低剂量的UV-B和定期干燥的交替处理下在固体BG11培养基上培养时，可得到40天后长到约3厘米长的深色且弯曲的丝状藻。此外，在缺氮的固体BG11培养基（BG11_（0））上的毛状体培养表明，氮的有效性可能影响新开发的拟南芥的颜色和润滑性。这项研究为将来的鞭毛猪笼草的人工栽培提供了有希望的技术。

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