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首页> 外文期刊>Applied Microbiology >Enhanced Inactivation of Salmonella and Pseudomonas Biofilms on Stainless Steel by Use of T-128, a Fresh-Produce Washing Aid, in Chlorinated Wash Solutions
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Enhanced Inactivation of Salmonella and Pseudomonas Biofilms on Stainless Steel by Use of T-128, a Fresh-Produce Washing Aid, in Chlorinated Wash Solutions

机译：通过使用T-128（一种新鲜产品洗涤助剂）在氯化洗涤溶液中增强不锈钢上沙门氏菌和假单胞菌生物膜的失活

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The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash solutions dominated by hypochlorous acid. Biofilms were formed statically on stainless steel coupons suspended in 2% lettuce extract after inoculation with Salmonella enterica serovar Thompson or Newport or with Pseudomonas fluorescens . Coupons with biofilms were washed in chlorine solutions (0, 0.5, 1, 2, 5, 10, or 20 mg/liter at pH 6.5, 5.0 and 2.9), with or without T-128, and with increasing loads of organic matter (0, 0.25, 0.5, 0.75, or 1.0% lettuce extract). Cell populations on coupons were dispersed using intermittent, pulsed ultrasonication and vortexing and enumerated by colony counts on XLT-4 or Pseudomonas agars. Cell responses to fluorescent viability staining of biofilm treatment washing solutions were examined using confocal laser scanning microscopy. Results showed that 0.1% T-128 (without chlorine) reduced P. fluorescens biofilm populations by 2.5 log_(10) units but did not reduce Salmonella populations. For both Salmonella and Pseudomonas , the sanitizing effect of free chlorine (1.0 to 5.0 mg/liter) was enhanced ( P < 0.05) when it was combined with T-128. Application of T-128 decreased the free chlorine depletion rate caused by increasing organic matter in wash waters and significantly ( P < 0.05) augmented inactivation of bacteria in biofilms compared to treatments without T-128. Image analysis of surfaces stained with SYTO and propidium iodide corroborate the cultural assay results showing that T-128 can aid in reducing pathogen viability in biofilms and thus can aid in sanitizing stainless steel contact surfaces during processing of fresh-cut produce.
机译：在有机物负荷增加的条件下，评估了洗涤助剂T-128（通常被认为是安全的[GRAS]配方，主要由磷酸和丙二醇组成）对不锈钢生物膜中沙门氏菌和假单胞菌种群失活的影响。次氯酸为主的氯化洗涤溶液。用肠沙门氏菌血清型汤普森或纽波特或荧光假单胞菌接种后，在悬浮于2％生菜提取物中的不锈钢试样上静态形成生物膜。在有或没有T-128且有机物负荷增加的情况下，在含氯溶液（pH 6.5、5.0和2.9的0、0.5、1、2、5、10或20 mg / L）中洗涤带有生物膜的优惠券。 0、0.25、0.5、0.75或1.0％的生菜提取物。使用间歇，脉冲超声和涡旋分散在附连体上的细胞群，并通过XLT-4或假单胞菌琼脂上的菌落计数进行计数。使用共聚焦激光扫描显微镜检查细胞对生物膜处理洗涤液的荧光活力染色的反应。结果表明，0.1％的T-128（不含氯）使荧光假单胞菌的生物膜种群减少了2.5 log_（10）个单位，但并未减少沙门氏菌的种群。对于沙门氏菌和假单胞菌，当与T-128结合使用时，其游离氯（1.0至5.0 mg / L）的消毒效果得到了增强（P <0.05）。与不使用T-128的处理相比，T-128的使用降低了由洗涤水中有机物增加引起的自由氯耗竭率，并且显着（P <0.05）增加了生物膜中细菌的失活。用SYTO和碘化丙啶染色的表面的图像分析证实了文化检测结果，该结果表明T-128可以帮助减少生物膜中的病原体生存力，因此可以帮助在鲜切农产品加工过程中消毒不锈钢接触表面。

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