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首页> 外文期刊>Applied Microbiology >The Burkholderia contaminans MS14 ocfC Gene Encodes a Xylosyltransferase for Production of the Antifungal Occidiofungin
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The Burkholderia contaminans MS14 ocfC Gene Encodes a Xylosyltransferase for Production of the Antifungal Occidiofungin

机译：Burkholderia contaminans MS14 ocfC基因编码木糖基转移酶，用于生产抗真菌的Occidiofungin。

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Burkholderia contaminans strain MS14 produces the antifungal compound occidiofungin, which is responsible for significant antifungal activities against a broad range of plant and animal fungal pathogens. Occidiofungin is a cyclic glycolipopeptide made up of eight amino acids and one xylose. A 56-kb ocf gene cluster was determined to be essential for occidiofungin production. In this study, the ocfC gene, which is located downstream of ocfD and upstream of the ocfB gene in the ocf gene cluster, was examined. Antifungal activity of the ocfC gene mutant MS14KC1 was reduced against the indicator fungus Geotrichum candidum compared with that of the wild-type strain. Furthermore, the analysis of the protein sequence suggests that the ocfC gene encodes a glycosyltransferase. Biochemical analyses using nuclear magnetic resonance (NMR) and mass spectroscopy revealed that the ocfC mutant produced the occidiofungin without the xylose. The purified ocfC mutant MS14KC1 product had a level of bioactivity similar to that of the wild-type product. The revertant MS14KC1-R of the ocfC mutant produced the same antifungal activity level on plate assays and the same antifungal compound based on high-performance liquid chromatography (HPLC) and mass spectroscopy analysis as wild-type strain MS14. Collectively, the study demonstrates that the ocfC gene encodes a glycosyltransferase responsible to add a xylose to the occidiofungin molecule and that the presence of the xylose is not important for antifungal activity against Candida species. The finding provides a novel variant for future studies aimed at evaluating its use for inhibiting clinical and agricultural fungi, and the finding could also simplify the chemical synthesis of occidiofungin variants.
机译：传染性伯克霍尔德氏菌菌株MS14产生抗真菌化合物occidiofungin，对多种动植物真菌病原体具有重要的抗真菌活性。 Occidiofungin是一种环状糖脂肽，由八个氨基酸和一个木糖组成。确定了一个56 kb的ocf基因簇，对生产occidiofungin至关重要。在这项研究中，检查了ocfC基因，该基因位于ocfD下游，位于ocf基因簇中ocfB基因的上游。与野生型菌株相比，ocfC基因突变体MS14KC1的抗真菌活性对念珠菌Geodrichum candidum有所降低。此外，对蛋白质序列的分析表明，ocfC基因编码糖基转移酶。使用核磁共振（NMR）和质谱的生化分析表明ocfC突变体产生了没有木糖的occidiofungin。纯化的ocfC突变体MS14KC1产品的生物活性水平与野生型产品相似。 ocfC突变体的回复体MS14KC1-R在平板分析中产生的抗真菌活性水平与基于高效液相色谱（HPLC）和质谱分析的抗真菌化合物产生的抗真菌活性水平与野生型菌株MS14相同。总的来说，该研究表明ocfC基因编码一个糖基转移酶，负责将木糖添加到occidiofungin分子中，并且木糖的存在对于针对念珠菌的抗真菌活性并不重要。该发现为将来的研究提供了一种新颖的变异体，旨在评估其在抑制临床和农业真菌中的用途，并且该发现还可以简化奥昔芬净变异体的化学合成。

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