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Effect of Shaking Speed and Type of Closure on Shake Flask Cultures

机译:振荡速度和闭合方式对振荡烧瓶培养的影响

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Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth of Escherichia coli B in a rich medium and production of candidin by Streptomyces viridoflavus were the test systems used. Flasks shaken at 230 to 385 rev/min gave sulfite oxidation rates of 1 to 8 mmoles of oxygen per liter per min over a useful working volume range (40 to 150 ml in 300-ml flasks). These rates are as high as those obtained in agitated fermentors under usual operating conditions.
机译:摇瓶中微生物的生长和微生物产物的生物合成可能受到操作条件的限制,操作条件会提供不足的氧气。描述了通过使用带有垫子式封闭物的标准带挡板烧瓶并以相对较高的速度摇晃来满足测试生物的氧气需求的方法。大肠杆菌B在丰富培养基中的生长和绿链霉菌链霉菌素的生产念珠菌素是所使用的测试系统。在有用的工作体积范围内(300毫升烧瓶中40到150毫升),以230至385转/分钟的速度摇动烧瓶,得到的亚硫酸盐氧化速率为每升每分钟1至8毫摩尔氧气。这些速率与在常规操作条件下在搅拌发酵罐中获得的速率一样高。

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