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Evaluating the Membrane Fecal Coliform Test by Using Escherichia coli as the Indicator Organism

机译:以大肠杆菌为指示生物评估膜粪大肠菌群测试

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The fecal coliform membrane filter method (MFC) currently used in water pollution analysis was evaluated by using two strains of Escherichia coli, a known fecal coliform, as the indicator organism. A large relative error in the results obtained with this method was found to be dependent upon the brand of membrane filter employed, the medium, and the temperature of incubation. MFC densities varied between 10 and 60% of the densities determined by means of total bacteria counts and total coliform counts performed at 35 C. Due to the large relative error encountered, the MFC method cannot be recommended as an analytical tool for the laboratory enumeration of E. coli. The results do show that the MFC method can be used at 35 C for enumeration of E. coli and for differential counts of E. coli and Enterobacter aerogenes.
机译:目前,水污染分析中使用的粪便大肠菌膜滤膜法(MFC)是通过使用两种大肠杆菌(一种已知的粪便大肠菌)作为指示生物进行评估的。发现用这种方法获得的结果中的较大相对误差取决于所用的膜滤器的品牌,培养基和培养温度。 MFC的密度在35 C下通过细菌总数和大肠菌群总数确定的密度的10%到60%之间变化。由于遇到的相对误差较大,因此不建议将MFC方法推荐为实验室枚举的分析工具大肠杆菌。结果确实表明,MFC方法可在35 C下用于大肠杆菌的计数以及大肠杆菌和产气肠杆菌的计数。

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