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New Gas Chromatographic Characterization Procedure: Preliminary Studies on Some Pseudomonas Species

机译：气相色谱法表征的新方法：一些假单胞菌属物种的初步研究

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摘要

Strains of saccharolytic and nonsaccharolytic Pseudomonas species were examined by a new single-step gas chromatographic characterization procedure. Cells were digested in a methanolic solution of tetramethylammonium hydroxide pentahydrate, and the digestates were subjected to gas-liquid chromatographic analysis. The chromatograms were examined for similarities and differences in their overall patterns. A single component was defined for use as an internal qualitative and quantitative standardizing component in order to develop relative retention time-relative peak height profiles of each organism. Comparison of these profiles enabled the characterization of strains of Pseudomonas aeruginosa, P. putida, P. cepacia, P. pseudomallei, P. stutzeri, P. pseudoalcaligenes, P. alcaligenes, P. diminuta, P. denitrificans, and P. acidovorans. The P. maltophilia and P. putrefaciens digestates showed chromatograms which were superficially similar yet easily distinguished as belonging to different species. The chromatograms of these two organisms were very different from those of other pseudomonads.
机译：通过新的单步气相色谱表征程序检查了糖化假单胞菌和非糖化假单胞菌的菌株。在五水合四甲基氢氧化铵的甲醇溶液中消化细胞，并对消化物进行气-液色谱分析。检查色谱图的总体模式是否相同和不同。为了发展每个生物体的相对保留时间相对于峰高的轮廓，定义了一个单一组分用作内部定性和定量标准化组分。这些概况的比较使得能够表征铜绿假单胞菌，恶臭假单胞菌，洋葱假单胞菌，假苹果假单胞菌，stutzeri，假拟假拟杯假单胞菌，产碱假单胞菌，diminuta假单胞菌，反硝化假单胞菌和嗜酸假单胞菌的菌株。嗜疟假单胞菌和腐烂假单胞菌的消化物显示的色谱图表面相似，但很容易区分为不同物种。这两种生物的色谱图与其他假单胞菌的色谱图非常不同。

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