Adaptation and Infection of Mouse Bone Marrow (JLS-V9) Cells in Suspension Culture for Production of Rauscher Leukemia Virus

Howard M. Hodge; Frederick Klein; Alok K. Bandyopadhyay; Orson R. Robinson; George P. Shibley

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Adaptation and Infection of Mouse Bone Marrow (JLS-V9) Cells in Suspension Culture for Production of Rauscher Leukemia Virus

机译：悬浮培养小鼠骨髓（JLS-V9）细胞对生产罗氏白血病病毒的适应和感染

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JLS-V9 mouse bone marrow cells were readily adapted to suspension culture, chronically infected with Rauscher leukemia virus (RLV), and subsequently grown in 7.5- and 14-liter New Brunswick fermentors. The suspension-type cell system can be modified to produce virus with clearly defined properties, such as high ribonucleic acid-dependent deoxyribonucleic acid polymerase (RDDP) activity, high particle count, and high infectious particle count. Biological and biophysical properties of suspension-produced RLV were not affected by concentration and purification employing continuous-flow and rate-zonal centrifugation procedures. The RDDP assay was standardized and showed a linear incorporation of 3H-thymidine 5′-monophosphate (3H-TMP) up to 30 min. Further characterization indicated that a high percentage of 3H-TMP incorporation was due to RDDP.

机译：JLS-V9小鼠骨髓细胞易于适应悬浮培养，长期感染劳氏白血病病毒（RLV），随后在7.5升和14升New Brunswick发酵罐中生长。可以对悬浮型细胞系统进行修饰，以产生具有明确定义的特性的病毒，例如高核糖核酸依赖性脱氧核糖核酸聚合酶（RDDP）活性，高颗粒数和高感染性颗粒数。悬浮液生产的RLV的生物学和生物物理特性不受使用连续流和速率分区离心程序进行浓缩和纯化的影响。 RDDP测定法经过标准化，显示3H-胸苷5'-单磷酸酯（3H-TMP）线性掺入直至30分钟。进一步的表征表明，高比例的3H-TMP掺入归因于RDDP。

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《Applied Microbiology》 |1974年第1期|共8页
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Howard M. Hodge; Frederick Klein; Alok K. Bandyopadhyay; Orson R. Robinson; George P. Shibley;
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1. Enhanced production of Rauscher leukemia virus after infection of high-passaged JLS-V9 cells. [J] . A Perry, O R Robinson, G P Shibley Applied and Environmental Microbiology . 1978,第3期

机译：感染高传代的JLS-V9细胞后，劳氏白血病病毒的产生增加。
2. Retrovirus gene expression during the cell cycle. I. Virus production, synthesis, and expression of viral proteins in Rauscher murine leukemia virus-infected mouse cells. [J] . I Balazs, J Caldarella Journal of Virology . 1981,第3期

机译：细胞周期中的逆转录病毒基因表达。 I.病毒生产，合成和病毒蛋白在Rauscher鼠白血病病毒感染的小鼠细胞中的表达。
3. Retrovirus gene expression during the cell cycle. I. Virus production, synthesis, and expression of viral proteins in Rauscher murine leukemia virus-infected mouse cells. [J] . I Balazs, J Caldarella Journal of Virology . 1981,第3期

机译：细胞周期中的逆转录病毒基因表达。 I.病毒生产，合成和病毒蛋白在Rauscher鼠白血病病毒感染的小鼠细胞中的表达。
4. ADAPTATION OF VERO CELLS TO SUSPENSION CULTURE AND RABIES VIRUS PRODUCTION ON DIFFERENT SERUM FREE MEDIA [C] . Samia Rourou, Meriem Ben Zakkour, Hela Kallel Conference on vaccine technology VI . 2018

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5. Evaluating the role of dendritic cells in host immune response during human T cell leukemia virus type 1 (HTLV-1) infection using a transgenic mouse model system [D] . Rahman, Saifur 2010

机译：使用转基因小鼠模型系统评估树突状细胞在人T细胞白血病病毒1型（HTLV-1）感染期间宿主免疫反应中的作用
6. Adaptation and Infection of Mouse Bone Marrow (JLS-V9) Cells in Suspension Culture for Production of Rauscher Leukemia Virus [O] . Howard M. Hodge, Frederick Klein, Alok K. Bandyopadhyay, 1974

机译：悬浮培养小鼠骨髓（JLS-V9）细胞对生产罗氏白血病病毒的适应和感染
7. Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation. [O] . Mary K McCarthy, Megan C Procario, Carol A Wilke, 2015

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机译：小鼠I型干扰素对体外培养的小鼠骨髓细胞和腹膜渗出细胞的影响

Adaptation and Infection of Mouse Bone Marrow (JLS-V9) Cells in Suspension Culture for Production of Rauscher Leukemia Virus

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