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首页> 外文期刊>Applied Microbiology >Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost
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Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost

机译：嗜热生物堆富集的纤维素分解财团中细菌和糖基水解酶家族48基因的多样性。

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The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of microbial cellulose degradation. In this work we identify predominant organisms in three cellulolytic enrichment cultures with thermophilic compost as an inoculum. Community structure based on 16S rRNA gene clone libraries featured extensive representation of clostridia from cluster III, with minor representation of clostridial clusters I and XIV and a novel Lutispora species cluster. Our studies reveal different levels of 16S rRNA gene diversity, ranging from 3 to 18 operational taxonomic units (OTUs), as well as variability in community membership across the three enrichment cultures. By comparison, glycosyl hydrolase family 48 (GHF48) diversity analyses revealed a narrower breadth of novel clostridial genes associated with cultured and uncultured cellulose degraders. The novel GHF48 genes identified in this study were related to the novel clostridia Clostridium straminisolvens and Clostridium clariflavum , with one cluster sharing as little as 73% sequence similarity with the closest known relative. In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured species.
机译：具有高纤维素分解活性的新型微生物群落从自然界中的富集可用于鉴定新型生物和新型功能，从而增强对微生物纤维素降解的基本认识。在这项工作中，我们以嗜热堆肥作为接种物，鉴定了三种纤维素富集培养中的主要微生物。基于16S rRNA基因克隆文库的社区结构以簇III中梭状芽胞杆菌的广泛代表为特征，梭菌I和XIV梭菌属的一个小代表以及一个新的Lutispora物种簇的代表。我们的研究揭示了不同水平的16S rRNA基因多样性，范围从3到18个操作生物分类单位（OTU），以及三种富集文化中社区成员的变异性。相比之下，糖基水解酶家族48（GHF48）多样性分析显示，与培养的和未培养的纤维素降解物相关的新型梭菌基因的范围更窄。在这项研究中鉴定出的新型GHF48基因与新型梭状梭状芽胞杆菌和克拉氏梭状芽胞杆菌有关，一个簇与最接近的亲戚的序列相似性低至73％。总共向已知物种的35个基因中添加了14个新的GHF48基因序列。

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