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首页> 外文期刊>Applied Microbiology >Neutrophilic Iron-Oxidizing “Zetaproteobacteria” and Mild Steel Corrosion in Nearshore Marine Environments
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Neutrophilic Iron-Oxidizing “Zetaproteobacteria” and Mild Steel Corrosion in Nearshore Marine Environments

机译：中性铁氧化“ Zetaproteobacteria”和近海海洋环境中的轻度钢腐蚀

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Microbiologically influenced corrosion (MIC) of mild steel in seawater is an expensive and enduring problem. Little attention has been paid to the role of neutrophilic, lithotrophic, iron-oxidizing bacteria (FeOB) in MIC. The goal of this study was to determine if marine FeOB related to Mariprofundus are involved in this process. To examine this, field incubations and laboratory microcosm experiments were conducted. Mild steel samples incubated in nearshore environments were colonized by marine FeOB, as evidenced by the presence of helical iron-encrusted stalks diagnostic of the FeOB Mariprofundus ferrooxydans , a member of the candidate class “ Zetaproteobacteria. ” Furthermore, Mariprofundus -like cells were enriched from MIC biofilms. The presence of Zetaproteobacteria was confirmed using a Zetaproteobacteria -specific small-subunit (SSU) rRNA gene primer set to amplify sequences related to M. ferrooxydans from both enrichments and in situ samples of MIC biofilms. Temporal in situ incubation studies showed a qualitative increase in stalk distribution on mild steel, suggesting progressive colonization by stalk-forming FeOB. We also isolated a novel FeOB, designated Mariprofundus sp. strain GSB2, from an iron oxide mat in a salt marsh. Strain GSB2 enhanced uniform corrosion from mild steel in laboratory microcosm experiments conducted over 4 days. Iron concentrations (including precipitates) in the medium were used as a measure of corrosion. The corrosion in biotic samples (7.4 ± 0.1 mM) was significantly higher than that in abiotic controls (5.0 ± 0.1 mM). These results have important implications for the role of FeOB in corrosion of steel in nearshore and estuarine environments. In addition, this work shows that the global distribution of Zetaproteobacteria is far greater than previously thought.
机译：海水对低碳钢的微生物影响腐蚀（MIC）是一个昂贵且持久的问题。 MIC中的嗜中性，锂养性，铁氧化细菌（FeOB）的作用很少关注。这项研究的目的是确定与Mariprofundus有关的海洋FeOB是否参与此过程。为了检验这一点，进行了现场培养和实验室缩影实验。海洋FeOB定殖了在近岸环境中培养的低碳钢样本，通过诊断FeOB Mariprofundus ferrooxydans（候选类Zetaproteobacteria的成员）中存在螺旋铁包裹的茎杆来证明。而且，类似Mariprofundus的细胞从MIC生物膜中富集了。使用Zetaproteobacteria特异的小亚基（SSU）rRNA基因引物确定了Zetaproteobacteria的存在，该引物被设置为从MIC生物膜的富集和原位样品中扩增与四氧化四铁笼草相关的序列。时间原位温育研究表明，低碳钢上茎的分布在质量上有所增加，表明通过形成茎的FeOB逐渐定居。我们还分离了一种新型的FeOB，命名为Mariprofundus sp。 GSB2菌株，来自盐沼中的氧化铁垫。在4天的实验室缩影实验中，GSB2应变增强了低碳钢的均匀腐蚀。介质中的铁浓度（包括沉淀物）用作腐蚀的量度。生物样品中的腐蚀（7.4±0.1 mM）明显高于非生物样品中的腐蚀（5.0±0.1 mM）。这些结果对FeOB在近岸和河口环境中的钢腐蚀中的作用具有重要意义。此外，这项工作表明，Zetaproteobacteria的全球分布远比以前认为的要大。

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