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首页> 外文期刊>Applied Microbiology >Response of Fatty Acid Synthesis Genes to the Binding of Human Salivary Amylase by Streptococcus gordonii
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Response of Fatty Acid Synthesis Genes to the Binding of Human Salivary Amylase by Streptococcus gordonii

机译：脂肪酸合成基因对戈登氏链球菌人唾液淀粉酶结合的响应

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Streptococcus gordonii , an important primary colonizer of dental plaque biofilm, specifically binds to salivary amylase via the surface-associated amylase-binding protein A (AbpA). We hypothesized that a function of amylase binding to S. gordonii may be to modulate the expression of chromosomal genes, which could influence bacterial survival and persistence in the oral cavity. Gene expression profiling by microarray analysis was performed to detect genes in S. gordonii strain CH1 that were differentially expressed in response to the binding of purified human salivary amylase versus exposure to purified heat-denatured amylase. Selected genes found to be differentially expressed were validated by quantitative reverse transcription-PCR (qRT-PCR). Five genes from the fatty acid synthesis (FAS) cluster were highly (10- to 35-fold) upregulated in S. gordonii CH1 cells treated with native amylase relative to those treated with denatured amylase. An abpA -deficient strain of S. gordonii exposed to amylase failed to show a response in FAS gene expression similar to that observed in the parental strain. Predicted phenotypic effects of amylase binding to S. gordonii strain CH1 (associated with increased expression of FAS genes, leading to changes in fatty acid synthesis) were noted; these included increased bacterial growth, survival at low pH, and resistance to triclosan. These changes were not observed in the amylase-exposed abpA -deficient strain, suggesting a role for AbpA in the amylase-induced phenotype. These results provide evidence that the binding of salivary amylase elicits a differential gene response in S. gordonii , resulting in a phenotypic adjustment that is potentially advantageous for bacterial survival in the oral environment.
机译：戈登链球菌是牙菌斑生物膜的重要主要定居者，它通过与表面相关的淀粉酶结合蛋白A（AbpA）与唾液淀粉酶特异性结合。我们假设淀粉酶结合戈登氏链球菌的功能可能是调节染色体基因的表达，这可能会影响细菌的存活和在口腔中的持久性。通过微阵列分析进行基因表达谱分析以检测戈登氏链球菌CH1菌株中的基因，这些基因响应于纯化的人唾液淀粉酶的结合与暴露于纯化的热变性淀粉酶的反应而差异表达。通过定量逆转录PCR（qRT-PCR）验证发现的差异表达的选定基因。相对于用变性淀粉酶处理的那些基因，来自脂肪酸合成（FAS）簇的五个基因在用天然淀粉酶处理的戈登酵母CH1细胞中高度上调（10到35倍）。暴露于淀粉酶的戈登氏链球菌abpA缺陷菌株未能在FAS基因表达中显示出与亲本菌株相似的应答。注意到了淀粉酶与戈氏链球菌CH1菌株结合的预期表型效应（与FAS基因表达的增加相关，导致脂肪酸合成的改变）;这些包括增加细菌生长，在低pH下存活以及对三氯生具有抗性。在暴露于淀粉酶的abpA缺陷菌株中未观察到这些变化，表明AbpA在淀粉酶诱导的表型中起作用。这些结果提供了证据，唾液淀粉酶的结合在戈登链球菌中引起差异基因应答，从而导致表型调节，这可能有利于细菌在口腔环境中的存活。

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