• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Phenotypic Switching in Pseudomonas brassicacearum Involves GacS- and GacA-Dependent Rsm Small RNAs
【24h】

Phenotypic Switching in Pseudomonas brassicacearum Involves GacS- and GacA-Dependent Rsm Small RNAs

机译：黄铜假单胞菌的表型转换涉及GacS和GacA依赖性Rsm小RNA。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The plant-beneficial bacterium Pseudomonas brassicacearum forms phenotypic variants in vitro as well as in planta during root colonization under natural conditions. Transcriptome analysis of typical phenotypic variants using microarrays containing coding as well as noncoding DNA fragments showed differential expression of several genes relevant to secondary metabolism and of the small RNA (sRNA) genes rsmX , rsmY , and rsmZ . Naturally occurring mutations in the gacS-gacA system accounted for phenotypic switching, which was characterized by downregulation of antifungal secondary metabolites (2,4-diacetylphloroglucinol and cyanide), indoleacetate, exoenzymes (lipase and protease), and three different N -acyl-homoserine lactone molecules. Moreover, in addition to abrogating these biocontrol traits, gacS and gacA mutations resulted in reduced expression of the type VI secretion machinery, alginate biosynthesis, and biofilm formation. In a gacA mutant, the expression of rsmX was completely abolished, unlike that of rsmY and rsmZ . Overexpression of any of the three sRNAs in the gacA mutant overruled the pleiotropic changes and restored the wild-type phenotypes, suggesting functional redundancy of these sRNAs. In conclusion, our data show that phenotypic switching in P. brassicacearum results from mutations in the gacS-gacA system.
机译：在自然条件下根部定植期间，植物有益细菌黄铜假单胞菌在体外以及在植物中形成表型变体。使用包含编码和非编码DNA片段的微阵列对典型表型变异进行转录组分析，结果表明与次级代谢相关的几个基因和小RNA（sRNA）基因rsmX，rsmY和rsmZ的差异表达。 gacS-gacA系统中自然发生的突变是表型转换的特征，其特征是抗真菌次级代谢产物（2,4-二乙酰基间苯三酚和氰化物），吲哚乙酸酯，外酶（脂肪酶和蛋白酶）和三种不同的N-酰基-高丝氨酸内酯分子。此外，除了废除这些生物控制性状外，gacS和gacA突变还导致VI型分泌机制的表达减少，藻酸盐的生物合成和生物膜形成。在gacA突变体中，与rsmY和rsmZ不同，rsmX的表达被完全消除。 gacA突变体中三个sRNA的任何一个的过表达都推翻了多效性变化并恢复了野生型表型，表明这些sRNA的功能冗余。总之，我们的数据表明，黄铜假单胞菌的表型转换是由gacS-gacA系统的突变引起的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号