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首页> 外文期刊>Applied Microbiology >CelR, an Ortholog of the Diguanylate Cyclase PleD of Caulobacter, Regulates Cellulose Synthesis in Agrobacterium tumefaciens
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CelR, an Ortholog of the Diguanylate Cyclase PleD of Caulobacter, Regulates Cellulose Synthesis in Agrobacterium tumefaciens

机译：CelR，杆状细菌的双鸟苷酸环化酶PleD的直系同源基因，调节根癌农杆菌中的纤维素合成

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Cellulose fibrils play a role in attachment of Agrobacterium tumefaciens to its plant host. While the genes for cellulose biosynthesis in the bacterium have been identified, little is known concerning the regulation of the process. The signal molecule cyclic di-GMP (c-di-GMP) has been linked to the regulation of exopolysaccharide biosynthesis in many bacterial species, including A. tumefaciens . In this study, we identified two putative diguanylate cyclase genes, celR ( atu1297 ) and atu1060 , that influence production of cellulose in A. tumefaciens . Overexpression of either gene resulted in increased cellulose production, while deletion of celR , but not atu1060 , resulted in decreased cellulose biosynthesis. celR overexpression also affected other phenotypes, including biofilm formation, formation of a polar adhesion structure, plant surface attachment, and virulence, suggesting that the gene plays a role in regulating these processes. Analysis of celR and Δ cel mutants allowed differentiation between phenotypes associated with cellulose production, such as biofilm formation, and phenotypes probably resulting from c-di-GMP signaling, which include polar adhesion, attachment to plant tissue, and virulence. Phylogenetic comparisons suggest that species containing both celR and celA , which encodes the catalytic subunit of cellulose synthase, adapted the CelR protein to regulate cellulose production while those that lack celA use CelR, called PleD, to regulate specific processes associated with polar localization and cell division.
机译：纤维素原纤维在根癌土壤杆菌对其植物宿主的附着中起作用。尽管已经鉴定出细菌中纤维素生物合成的基因，但关于该过程的调控知之甚少。信号分子环状di-GMP（c-di-GMP）与许多细菌物种（包括根癌农杆菌）中胞外多糖的生物合成调控有关。在这项研究中，我们确定了两个假定的双鸟苷酸环化酶基因，celR（atu1297）和atu1060，它们影响根癌农杆菌中纤维素的产生。任一基因的过表达导致纤维素产量增加，而celR的缺失而不是atu1060的缺失导致纤维素生物合成的减少。 celR的过表达也影响其他表型，包括生物膜形成，极性粘附结构的形成，植物表面附着和毒力，这表明该基因在调节这些过程中起作用。 celR和Δcel突变体的分析允许区分与纤维素生产相关的表型（例如生物膜形成）和可能由c-di-GMP信号转导的表型，包括极性粘附，对植物组织的附着和毒力。系统发育比较表明，同时包含celR和celA的物种（它们编码纤维素合酶的催化亚基）使CelR蛋白调节纤维素的生产，而那些缺乏celA的物种则使用称为PleD的CelR来调节与极性定位和细胞分裂有关的特定过程。 。

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