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Occurrence of Potentially Human-Pathogenic Escherichia coli O103 in Norwegian Sheep

机译：挪威绵羊中潜在的人类致病性大肠杆菌O103的发生

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The investigation of an outbreak of hemorrhagic-uremic syndrome in Norway in 2006 indicated that the outbreak strain Escherichia coli O103:H25 could originate from sheep. A national survey of the Norwegian sheep population was performed, with the aim of identifying and describing a possible reservoir of potentially human-pathogenic E. coli O103, in particular of the H types 2 and 25. The investigation of fecal samples from 585 sheep flocks resulted in 1,222 E. coli O103 isolates that were analyzed for the presence of eae and stx genes, while a subset of 369 isolates was further examined for flagellar antigens (H typing), stx subtypes, bfpA , astA , and molecular typing by pulsed-field gel electrophoresis (PFGE). The total ovine E. coli O103 serogroup was genetically diverse by numbers of H types, virulotypes, and PFGE banding patterns identified, although a tendency of clustering toward serotypes was seen. The flocks positive for potentially human-pathogenic E. coli O103 were geographically widely distributed, and no association could be found with county or geographical region. The survey showed that eae -negative, stx -negative E. coli O103, probably nonpathogenic to humans, is very common in sheep, with 27.5% of flocks positive. Moreover, the study documented a low prevalence (0.7%) of potentially human-pathogenic Shiga toxin-producing E. coli O103:H2, while STEC O103:H25 was not detected. However, 3.1% and 5.8% of the flocks were positive for enteropathogenic E. coli O103 belonging to H types 2 and 25, respectively. These isolates are of concern as potential human pathogens by themselves but more importantly as possible precursors for human-pathogenic STEC.
机译：2006年在挪威进行的一次出血性尿毒症综合征暴发调查表明，大肠杆菌O103：H25暴发菌株可能源自绵羊。对挪威绵羊种群进行了一次全国调查，目的是识别和描述可能的人类致病性大肠杆菌O103，特别是2型和25型H大肠杆菌的潜在库。调查了585个羊群的粪便样本产生了1,222个大肠杆菌O103分离株，分析了eae和stx基因的存在，同时进一步检查了369个分离株的一个亚群的鞭毛抗原（H型），stx亚型，bfpA，astA和通过脉冲分选的分子分型场凝胶电泳（PFGE）。绵羊总大肠杆菌O103血清型在遗传上因H型，病毒型和PFGE条带模式的数量而异，尽管发现有向血清型聚集的趋势。潜在的人类致病性大肠杆菌O103呈阳性的鸡群在地理上分布广泛，与县或地理区域没有关联。调查显示，eae阴性，stx阴性的大肠杆菌O103（可能对人类无致病性）在绵羊中非常常见，羊群中有27.5％为阳性。此外，该研究表明，潜在的人致病志贺毒素生产大肠杆菌O103：H2的患病率较低（0.7％），而未检测到STEC O103：H25。但是，分别有3.1％和5.8％的鸡群对2型和25型H型肠致病性大肠杆菌O103呈阳性。这些分离株本身就被认为是潜在的人类病原体，但更重要的是作为人致病性STEC的可能前体。

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