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首页> 外文期刊>Applied Microbiology >Acquired Genetic Mechanisms of a Multiresistant Bacterium Isolated from a Treatment Plant Receiving Wastewater from Antibiotic Production
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Acquired Genetic Mechanisms of a Multiresistant Bacterium Isolated from a Treatment Plant Receiving Wastewater from Antibiotic Production

机译：从抗生素生产废水中获得污水处理厂分离的多耐药细菌的遗传机制。

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The external environment, particularly wastewater treatment plants (WWTPs), where environmental bacteria meet human commensals and pathogens in large numbers, has been highlighted as a potential breeding ground for antibiotic resistance. We have isolated the extensively drug-resistant Ochrobactrum intermedium CCUG 57381 from an Indian WWTP receiving industrial wastewater from pharmaceutical production contaminated with high levels of quinolones. Antibiotic susceptibility testing against 47 antibiotics showed that the strain was 4 to >500 times more resistant to sulfonamides, quinolones, tetracyclines, macrolides, and the aminoglycoside streptomycin than the type strain O. intermedium LMG 3301~(T). Whole-genome sequencing identified mutations in the Indian strain causing amino acid substitutions in the target enzymes of quinolones. We also characterized three acquired regions containing resistance genes to sulfonamides ( sul1 ), tetracyclines [ tet ( G ) and tetR ], and chloramphenicol/florfenicol ( floR ). Furthermore, the Indian strain harbored acquired mechanisms for horizontal gene transfer, including a type I mating pair-forming system (MPF_(I)), a MOB_(P) relaxase, and insertion sequence transposons. Our results highlight that WWTPs serving antibiotic manufacturing may provide nearly ideal conditions for the recruitment of resistance genes into human commensal and pathogenic bacteria.
机译：外部环境，特别是废水处理厂（WWTP），其中环境细菌会遇到人类共病和大量病原体，已被强调为可能产生抗生素耐药性的温床。我们从印度污水处理厂中分离了具有广泛耐药性的中间O虫CCUG 57381，该污水处理厂接受了受到喹诺酮类高污染药物生产的工业废水。对47种抗生素的药敏试验表明，该菌株对磺酰胺，喹诺酮类，四环素类，大环内酯类和氨基糖苷链霉素的抗性比中间菌株O. intermedium LMG 3301〜（T）高4到> 500倍。全基因组测序鉴定出印度菌株中的突变，该突变导致喹诺酮类靶酶中的氨基酸取代。我们还表征了三个包含对磺酰胺类（sul1），四环素[tet（G）和tetR]和氯霉素/氟苯尼考（floR）的抗性基因的获得区域。此外，印度菌株具有水平基因转移的获得机制，包括I型交配对形成系统（MPF_（I）），MOB_（P）松弛酶和插入序列转座子。我们的结果强调，为抗生素生产服务的污水处理厂可能为将抗性基因募集到人类共生病原菌中提供近乎理想的条件。

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