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首页> 外文期刊>Applied Microbiology >Sustainable Growth of Dehalococcoides mccartyi 195 by Corrinoid Salvaging and Remodeling in Defined Lactate-Fermenting Consortia
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Sustainable Growth of Dehalococcoides mccartyi 195 by Corrinoid Salvaging and Remodeling in Defined Lactate-Fermenting Consortia

机译：在定义的乳酸发酵联合体中通过类海藻蛋白抢救和重塑实现Dehalococcoidesides mccartyi 195的可持续增长

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Corrinoids are essential cofactors of reductive dehalogenases in Dehalococcoides mccartyi , an important bacterium in bioremediation, yet sequenced D. mccartyi strains do not possess the complete pathway for de novo corrinoid biosynthesis. Pelosinus sp. and Desulfovibrio sp. have been detected in dechlorinating communities enriched from contaminated groundwater without exogenous cobalamin corrinoid. To investigate the corrinoid-related interactions among key members of these communities, we constructed consortia by growing D. mccartyi strain 195 (Dhc195) in cobalamin-free, trichloroethene (TCE)- and lactate-amended medium in cocultures with Desulfovibrio vulgaris Hildenborough (DvH) or Pelosinus fermentans R7 (PfR7) and with both in tricultures. Only the triculture exhibited sustainable dechlorination and cell growth when a physiological level of 5,6-dimethylbenzimidazole (DMB), the lower ligand of cobalamin, was provided. In the triculture, DvH provided hydrogen while PfR7 provided corrinoids to Dhc195, and the initiation of dechlorination and Dhc195 cell growth was highly dependent on the growth of PfR7. Corrinoid analysis indicated that Dhc195 imported and remodeled the phenolic corrinoids produced by PfR7 into cobalamin in the presence of DMB. Transcriptomic analyses of Dhc195 showed the induction of the CbiZ-dependent corrinoid-remodeling pathway and BtuFCD corrinoid ABC transporter genes during corrinoid salvaging and remodeling. In contrast, another operon annotated to encode a putative iron/cobalamin ABC transporter (DET1174-DET1176) was induced when cobalamin was exogenously provided. Interestingly, a global upregulation of phage-related genes was observed when PfR7 was present. These findings provide insights into both the gene regulation of corrinoid salvaging and remodeling in Dhc195 when it is grown without exogenous cobalamin and microbe-to-microbe interactions in dechlorinating microbial communities.
机译：类固醇是生物修复中的重要细菌Dehalococcoides mccartyi中还原性脱卤酶的必需辅因子，但测序的D. mccartyi菌株不具备从头进行类固醇生物合成的完整途径。 Pelosinus sp。和Desulfovibrio sp。在没有外源性钴胺素类胡萝卜素的受污染地下水富集的脱氯社区中已检测到。为了研究这些社区关键成员之间与类固醇有关的相互作用，我们通过在无钴胺素，三氯乙烯（TCE）和乳酸修饰的培养基中与脱硫磺弧菌Hildenborough（DvH）共培养的D. mccartyi菌株195（Dhc195）构建了财团。 ）或发酵乳杆菌（Plosinus fermentans R7）（PfR7），并在三培养中同时使用。当提供生理水平的5，6-二甲基苯并咪唑（DMB）（钴胺素的较低配体）时，只有三培养物才显示出可持续的脱氯和细胞生长。在三培养中，DvH提供氢，而PfR7向Dhc195提供类皮质醇，脱氯和Dhc195细胞生长的开始高度依赖于PfR7的生长。皮质醇分析表明，Dhc195在DMB存在下将PfR7产生的酚类皮质醇导入并重塑为钴胺素。 Dhc195的转录组学分析显示，在挽救和重塑类固醇过程中，诱导了CbiZ依赖的类固醇重塑途径和BtuFCD类固醇ABC转运蛋白基因。相反，当外源提供钴胺素时，会诱导另一个标注编码假定的铁/钴胺素ABC转运蛋白的操纵子（DET1174-DET1176）。有趣的是，当存在PfR7时，观察到了噬菌体相关基因的整体上调。这些发现提供了对Dhc195中类固醇修复和重塑的基因调控的见解，当Dhc195在无外源钴胺素的作用下生长且在脱氯微生物群落中没有微生物之间的相互作用时。

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