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首页> 外文期刊>Applied Microbiology >Modification of Gene Expression and Virulence Traits in Streptococcus mutans in Response to Carbohydrate Availability
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Modification of Gene Expression and Virulence Traits in Streptococcus mutans in Response to Carbohydrate Availability

机译：变异链球菌中基因表达和毒力特征对碳水化合物可用性的响应的修改。

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The genetic and phenotypic responses of Streptococcus mutans , an organism that is strongly associated with the development of dental caries, to changes in carbohydrate availability were investigated. S. mutans UA159 or a derivative of UA159 lacking ManL, which is the EIIAB component (EIIAB~(Man)) of a glucose/mannose permease of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) and a dominant effector of catabolite repression, was grown in continuous culture to steady state under conditions of excess (100 mM) or limiting (10 mM) glucose. Microarrays using RNA from S. mutans UA159 revealed that 174 genes were differentially expressed in response to changes in carbohydrate availability ( P < 0.001). Glucose-limited cells possessed higher PTS activity, could acidify the environment more rapidly and to a greater extent, and produced more ManL protein than cultures grown with excess glucose. Loss of ManL adversely affected carbohydrate transport and acid tolerance. Comparison of the histidine protein (HPr) in S. mutans UA159 and the manL deletion strain indicated that the differences in the behaviors of the strains were not due to major differences in HPr pools or HPr phosphorylation status. Therefore, carbohydrate availability alone can dramatically influence the expression of physiologic and biochemical pathways that contribute directly to the virulence of S. mutans , and ManL has a profound influence on this behavior.
机译：研究了变形链球菌（一种与龋齿的发展密切相关的生物）对碳水化合物利用率变化的遗传和表型反应。生长了变形链球菌UA159或缺少ManL的UA159衍生物，它是磷酸烯醇丙酮酸：糖磷酸转移酶系统（PTS）的葡萄糖/甘露糖通透酶的EIIAB组分（EIIAB〜（Man））和分解代谢物阻遏的主要效应子。在葡萄糖过量（100 mM）或极限（10 mM）的条件下连续培养至稳态。使用来自变形链球菌UA159的RNA进行的微阵列分析显示，有174个基因在碳水化合物可利用性变化的响应中差异表达（P <0.001）。葡萄糖受限的细胞具有较高的PTS活性，与用过量葡萄糖培养的培养物相比，可以更快地酸化环境并在更大程度上酸化环境，并产生更多的ManL蛋白。失去ManL会对碳水化合物的转运和耐酸性产生不利影响。变形链球菌UA159和manL缺失菌株中组氨酸蛋白（HPr）的比较表明，菌株行为的差异并非归因于HPr池或HPr磷酸化状态的主要差异。因此，仅碳水化合物的可用性就可以极大地影响直接导致变形链球菌毒力的生理和生化途径的表达，而ManL对这种行为具有深远的影响。

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