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首页> 外文期刊>Applied Microbiology >Thermal Inactivation of Staphylococcal Enterotoxin B in Veronal Buffer
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Thermal Inactivation of Staphylococcal Enterotoxin B in Veronal Buffer

机译：Veronal缓冲液中的葡萄球菌肠毒素B的热灭活。

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The times and temperatures required to inactivate staphylococcal enterotoxin B were studied by use of the double-gel-diffusion technique to assay enterotoxin. Enterotoxin B (99 +% pure) was suspended in 0.04 M Veronal buffer, dispensed into borosilicate vials, and the vials were sealed and heated in an oil bath. An amount of 30 μg/ml of this toxin was reduced to less than 0.7 μg/ml in 103.0, 87.1, 70.5, 57.2, 39.1, 27.6, 16.4, and 12.0 min, respectively, at temperatures of 96, 99, 101.7, 104.4, 110, 115.6, 121, and 126.7 C. The end point for enterotoxin inactivation by gel diffusion was identical to that by intravenous injection of cats. Limited studies with crude enterotoxin B showed that the crude preparation was slightly more thermostable. The respective D values of crude and purified enterotoxin B were 64.5 and 52.3, 40.5 and 34.4, 29.7 and 23.5, 18.8 and 16.6, and 11.4 and 9.9 min at temperatures of 99, 104.4, 110, 115.6, and 121 C. The z value for purified enterotoxin B was 32.4 C. The experimental activation energy was 20,700 cal/g mole, standard enthalpy of activation at 120 C was 19,900 cal/g mole, standard entropy of activation at 120 C was -21.4 cal/g mole K, and the standard free energy of activation at 120 C was 28,200 cal/g mole.
机译：通过使用双凝胶扩散技术分析肠毒素来研究灭活葡萄球菌肠毒素B所需的时间和温度。将肠毒素B（99 +％的纯度）悬浮在0.04 M Veronal缓冲液中，分配到硼硅酸盐小瓶中，将小瓶密封并在油浴中加热。在96、99、101.7、104.4的温度下，分别在103.0、87.1、70.5、57.2、39.1、27.6、16.4和12.0分钟内将30μg/ ml的这种毒素的量减少至小于0.7μg/ ml。 ，110、115.6、121和126.7C。通过凝胶扩散灭活肠毒素的终点与通过猫的静脉注射灭活的终点相同。对粗肠毒素B的有限研究表明，粗制剂的热稳定性稍强。在99、104.4、110、115.6和121 C的温度下，粗制和纯化肠毒素B的D值分别为64.5和52.3、40.5和34.4、29.7和23.5、18.8和16.6、11.4和9.9分钟。z值纯化的肠毒素B的标准熵为32.4 C.实验活化能为20,700 cal / g摩尔，在120 C时的标准活化焓为19,900 cal / g摩尔，在120 C时的标准活化熵为-21.4 cal / g摩尔K，以及120℃下活化的标准自由能为28,200cal / g摩尔。

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