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Microbiological Assay for Organic Compounds in Seawater. II Distribution of Adenine, Uracil, and Threonine

机译:海水中有机化合物的微生物测定。 II腺嘌呤,尿嘧啶和苏氨酸的分布

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Biochemically deficient strains of Serratia marinorubra have been isolated with specific requirements for adenine, uracil, and threonine. Standard curves for dose to growth response have been obtained showing a linear sensitivity from 0.5 to 4.0 mg of adenine per liter of seawater, 0.1 to 2.0 mg of uracil per liter of seawater, and 0.5 to 10 mg of threonine per liter of seawater. These mutants have been used to test for the presence of their required metabolites in natural seawater samples from the Gulf of Mexico and adjacent bays. Of the three compounds under investigation, adenine was found in 10 samples, uracil in 2 samples, and threonine in none. The possible significance of these findings to the marine environment is discussed.
机译:已分离出对沙雷氏菌的生化缺陷型菌株,对腺嘌呤,尿嘧啶和苏氨酸有特定要求。已获得剂量对生长反应的标准曲线,显示线性灵敏度为每升海水0.5至4.0 mg腺嘌呤,每升海水0.1至2.0 mg尿嘧啶和每升海水0.5至10 mg苏氨酸。这些突变体已用于测试来自墨西哥湾和相邻海湾的天然海水样品中所需代谢产物的存在。在所研究的三种化合物中,在10个样品中发现了腺嘌呤,在2个样品中发现了尿嘧啶,而没有苏氨酸。讨论了这些发现对海洋环境的可能意义。

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