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首页> 外文期刊>Applied Microbiology >Limitations of Thioglycolate Broth as a Sterility Test Medium for Materials Exposed to Gaseous Ethylene Oxide
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Limitations of Thioglycolate Broth as a Sterility Test Medium for Materials Exposed to Gaseous Ethylene Oxide

机译：巯基乙酸盐肉汤作为无菌测试介质对气态环氧乙烷暴露的局限性

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Although ethylene oxide is a reliable sterilizer, the process may be limited by diffusion. Thus, situations may exist where microorganisms are protected from the sterilizing gas. It is possible that the exterior of a substance may be sterilized, whereas the interior is not. We investigated three general types of materials in which this limitation of diffusion could occur: the bore of glass and plastic tubing, the center of cotton balls, and plastic adhesive film/paper backing interface. These materials were contaminated as close to their geometric center as possible with Bacillus subtilis var. niger spores occluded in crystals of sodium chloride. After exposure of the contaminated materials (except aluminum foil) to ethylene oxide, thioglycolate broth (a standard sterility-test medium) indicated sterility, whereas Trypticase Soy Broth indicated nonsterility. It is likewise possible that aerobic microorganisms, surviving in or on material after exposure to dry heat or steam sterilization processes, would not be recovered by thioglycollate broth. Entrapped aerobic organisms will probably not grow out in the low oxygen tension zone of an anaerobic medium such as thioglycollate broth. It is recommended than an aerobic medium such as Trypticase Soy Broth be used concurrently with thioglycolate broth for sterility testing.
机译：尽管环氧乙烷是可靠的灭菌器，但该过程可能会受到扩散的限制。因此，可能存在保护微生物免受灭菌气体侵害的情况。物质的外部有可能被消毒，而内部没有被消毒。我们研究了可能会发生这种扩散限制的三种通用材料：玻璃和塑料管的内孔，棉球的中心以及塑料粘合膜/纸底衬的界面。这些材料被枯草芽孢杆菌（Bacillus subtilis var）污染到尽可能靠近其几何中心的程度。尼日尔孢子闭塞在氯化钠晶体中。将受污染的材料（铝箔除外）暴露于环氧乙烷后，巯基乙酸盐肉汤（标准无菌测试介质）显示无菌，而胰蛋白酶大豆肉汤则显示不无菌。同样可能的是，暴露于干热或蒸汽灭菌过程后残留在材料中或材料上的好氧微生物不会被巯基乙酸盐肉汤回收。被截留的好氧生物可能不会在厌氧培养基（如巯基乙酸盐肉汤）的低氧张力区中长出来。建议不要将有氧培养基（例如胰酶解大豆肉汤）与巯基乙酸盐肉汤同时使用，以进行无菌测试。

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