• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Factors Influencing Agnotobiotic Cultures of the Rumen Ciliate, Entodinium simplex
【24h】

Factors Influencing Agnotobiotic Cultures of the Rumen Ciliate, Entodinium simplex

机译：影响瘤胃纤毛，单纯虫的不育文化的因素

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nutrition of Entodinium simplex was studied, with foliage of bluegrass (Poa pratense), perennial ryegrass (Lolium perenne), and grains of wheat (Triticum vulgare) as substrates in agnotobiotic cultures. Entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. The concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. Autolysates and hydrolysates of mixed rumen protozoa stimulated growth. Protozoal extracts did not replace factors destroyed by autoclaving. Clarified rumen fluid assisted the cultivation of entodinia from small inocula but was detrimental to established cultures. Success of cultures was influenced by the medium used to grow the inoculum as well as by the medium inoculated. The results indicated that the composition of the bacterial population influences the growth of E. simplex.
机译：研究了简单的Entodinium的营养，其中以禾本科植物（Poa pratense），多年生黑麦草（Lolium perenne）和小麦粒（Triticum vulgare）的叶子为生源菌培养物。当基板被高压灭菌时，Entodinium生长不良。当用环氧乙烷蒸气对基板进行灭菌时，获得了更好的生长。环氧乙烷的浓度和水分的量会影响被处理基质的无菌性和营养充足性。瘤胃原生动物的自溶物和水解物刺激生长。原虫提取物不能替代被高​​压灭菌破坏的因子。澄清的瘤胃液有助于从小接种物中培养肠炎菌，但不利于已建立的培养物。培养的成功受到用于培养接种物的培养基以及接种培养基的影响。结果表明，细菌种群的组成影响了单纯大肠杆菌的生长。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号