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首页> 外文期刊>Applied Microbiology >Nonspecific Precipitation of Serum Proteins by Sodium Lauryl Sulfate in Agar Diffusion and Immunoelectrophoresis
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Nonspecific Precipitation of Serum Proteins by Sodium Lauryl Sulfate in Agar Diffusion and Immunoelectrophoresis

机译：月桂基硫酸钠在琼脂扩散和免疫电泳中非特异性沉淀血清蛋白

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摘要

The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin lines. These lines occurred when either Ionagar or agarose was used as the supporting gel and were not affected by the presence of urea and 2-mercaptoethanol. Analytic chemical tests confirmed that the precipitating agent is SLS, and staining techniques showed that the detergent precipitates both protein and lipoprotein components of whole serum. Multiple artifactual precipitin lines occurred with a wide variety of animal sera, and a single line formed with human 7S immunoglobulin. Hence, in agar diffusion studies in which SLS is present in the test system, these artifactual lines may be easily misinterpreted as true antigen-antibody precipitin reactions.
机译：最终浓度为0.1％或更高的阴离子型清洁剂月桂基硫酸钠（SLS）在琼脂扩散和免疫电泳中与全血清反应形成人为沉淀素系。当将爱奥那加或琼脂糖用作支持凝胶时，这些系就会出现，并且不受尿素和2-巯基乙醇的存在的影响。分析化学测试证实了沉淀剂是SLS，染色技术表明该洗涤剂沉淀了整个血清中的蛋白质和脂蛋白成分。多种动物血清中出现了多个人为的沉淀素系，而人类7S免疫球蛋白则形成了一条系。因此，在测试系统中存在SLS的琼脂扩散研究中，这些人为因素可能容易被误解为真正的抗原抗体沉淀蛋白反应。

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