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首页> 外文期刊>Applied Microbiology >Bacterial Community and “Candidatus Accumulibacter” Population Dynamics in Laboratory-Scale Enhanced Biological Phosphorus Removal Reactors
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Bacterial Community and “Candidatus Accumulibacter” Population Dynamics in Laboratory-Scale Enhanced Biological Phosphorus Removal Reactors

机译：实验室规模的增强型生物除磷反应器中细菌群落和“ Candidatus Accumulibacter”种群动态

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“ Candidatus Accumulibacter” and total bacterial community dynamics were studied in two lab-scale enhanced biological phosphorus removal (EBPR) reactors by using a community fingerprint technique, automated ribosomal intergenic spacer analysis (ARISA). We first evaluated the quantitative capability of ARISA compared to quantitative real-time PCR (qPCR). ARISA and qPCR provided comparable relative quantification of the two dominant “ Ca . Accumulibacter” clades (IA and IIA) detected in our reactors. The quantification of total “ Ca . Accumulibacter” 16S rRNA genes relative to that from the total bacterial community was highly correlated, with ARISA systematically underestimating “ Ca . Accumulibacter” abundance, probably due to the different normalization techniques applied. During 6 months of normal (undisturbed) operation, the distribution of the two clades within the total “ Ca . Accumulibacter” population was quite stable in one reactor while comparatively dynamic in the other reactor. However, the variance in the clade distribution did not appear to affect reactor performance. Instead, good EBPR activity was positively associated with the abundance of total “ Ca . Accumulibacter.” Therefore, we concluded that the different clades in the system provided functional redundancy. We disturbed the reactor operation by adding nitrate together with acetate feeding in the anaerobic phase to reach initial reactor concentrations of 10 mg/liter NO_(3)-N for 35 days. The reactor performance deteriorated with a concomitant decrease in the total “ Ca . Accumulibacter” population, suggesting that a population shift was the cause of performance upset after a long exposure to nitrate in the anaerobic phase.
机译：通过使用社区指纹技术，自动核糖体基因间隔分析（ARISA），在两个实验室规模的增强型生物除磷（EBPR）反应器中研究了“念珠菌”和总细菌群落动态。我们首先评估了ARISA与定量实时PCR（qPCR）相比的定量能力。 ARISA和qPCR提供了两个显着的“ Ca。在我们的反应堆中检测到了“积累细菌”进化枝（IA和IIA）。总“ Ca”的定量。相对于总细菌群落而言，Accumulibacter”的16S rRNA基因高度相关，ARISA系统地低估了“Ca。可能由于使用了不同的归一化技术而导致了“积累细菌”的丰富。在正常（不受干扰）操作的6个月中，两个进化枝在总Ca中的分布。在一个反应​​器中，积累的细菌数量相当稳定，而在另一反应器中则相对动态。但是，进化枝分布的变化似乎并未影响反应堆性能。取而代之的是，良好的EBPR活性与总Ca的丰度呈正相关。积累细菌。”因此，我们得出结论，系统中的不同分支提供了功能冗余。我们通过在厌氧相中添加硝酸盐和乙酸盐进料来扰乱反应器运行，从而在35天内达到10 mg / L NO_（3）-N的初始反应器浓度。反应器性能下降，总“ Ca”降低。积累细菌”，这表明在厌氧阶段长期暴露于硝酸盐后，种群转移是导致性能下降的原因。

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