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首页> 外文期刊>Applied Microbiology >Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil
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Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

机译：温度和捕食对土壤和粪肥改良土壤中肠炎沙门氏菌鼠伤寒沙门氏菌存活和invA表达的影响

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The effects of three temperatures (5, 15, and 25°C) on the survival of Salmonella enterica serovar Typhimurium in topsoil were investigated in small microcosms by three different techniques: plate counting, invA gene quantification, and invA mRNA quantification. Differences in survival were related to the effect of protozoan predation. Tetracycline-resistant Salmonella serovar Typhimurium was inoculated into soil and manure-amended soil at 1.5 × 10~(8) cells g soil~(?1). Population densities were determined by plate counting and by molecular methods and monitored for 42 days. Simultaneous extraction of RNA and DNA, followed by quantitative PCR, was used to investigate invA gene levels and expression. Analysis by these three techniques showed that Salmonella serovar Typhimurium survived better at 5°C. Comparing DNA and CFU levels, significantly higher values were determined by DNA-based techniques. invA mRNA levels showed a fast decrease in activity, with no detectable mRNA after an incubation period of less than 4 days in any of the soil scenarios. A negative correlation was found between Salmonell a serovar Typhimurium CFU levels and protozoan most probable numbers, and we propose the role of the predator-prey interaction as a factor to explain the die-off of the introduced strain by both culture- and DNA quantification-based methods. The results indicate that temperature, manure, and protozoan predation are important factors influencing the survival of Salmonella serovar Typhimurium in soil.
机译：通过三种不同的技术，通过板块计数，invA基因定量和invA mRNA定量三种不同的技术研究了三种温度（5、15和25°C）对小肠沙门氏菌血清型鼠伤寒生存的影响。生存差异与原生动物捕食的效果有关。将四环素抗性沙门氏菌鼠伤寒沙门氏菌以1.5×10〜（8）细胞/ g〜（？1）接种到土壤和粪肥改良的土壤中。通过平板计数和分子方法确定种群密度，并监测42天。同时提取RNA和DNA，然后进行定量PCR，用于研究invA基因的水平和表达。通过这三种技术的分析表明，鼠伤寒沙门氏菌在5°C时能更好地存活。比较DNA和CFU水平，通过基于DNA的技术确定了明显更高的值。在任何土壤情况下，孵育时间少于4天后，invA mRNA水平均显示出活性快速下降，而未检测到mRNA。沙门氏菌鼠伤寒沙门氏菌CFU水平与原生动物最可能的数量之间存在负相关关系，我们提出掠食者与猎物的相互作用是通过培养和DNA定量解释引入菌株死亡的一个因素。基于方法。结果表明温度，粪便和原生动物的捕食是影响土壤中鼠伤寒沙门氏菌存活的重要因素。

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