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首页> 外文期刊>Applied Microbiology >Bacterial Symbionts of the Brown Planthopper, Nilaparvata lugens (Homoptera: Delphacidae)
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Bacterial Symbionts of the Brown Planthopper, Nilaparvata lugens (Homoptera: Delphacidae)

机译：褐飞虱，Nilaparvata lugens（同翅目：Delphacidae）的细菌共生体。

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The brown planthopper ( Nilaparvata lugens St?l), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. There have also been many reports of a yeast-like symbiont of N. lugens , but little is known about the bacterial microbes. In this study, we examined the bacterial microbes in N. lugens and identified a total of 18 operational taxonomic units (OTUs) representing four phyla ( Proteobacteria , Firmicutes , Actinobacteria , and Bacteroidetes ) by sequencing and analyzing 16S rRNA gene libraries obtained from three populations of N. lugens , which were maintained on the rice varieties TN1, Mudgo, and ASD7. Several of the OTUs were similar to previously reported secondary symbionts of other insects, including an endosymbiont of the psyllid Glycapsis brimblecombei , an Asaia sp. found in the mosquito Anopheles stephensi , and Wolbachia , found in the mite Metaseiulus occidentalis . However, the species and numbers of the detected OTUs differed substantially among the N. lugens populations. Further, in situ hybridization analysis using digoxigenin-labeled probes indicated that OTU 1 was located in hypogastrium tissues near the ovipositor and ovary in biotype 1 insects, while OTU 2 was located in the front of the ovipositor sheath in biotype 2 insects. In addition, masses of bacterium-like organisms were observed in the tubes of salivary sheaths in rice plant tissues that the insects had fed upon. The results provide indications of the diversity of the bacterial microbes harbored by the brown planthopper and of possible associations between specific bacterial microbes and biotypes of N. lugens .
机译：已经鉴定出稻瘟病最具破坏性的褐飞虱（Nilaparvata lugens St？l），包括对以前具有抗性的水稻品种具有高毒力的生物型。也有许多关于黄褐猪笼草的酵母样共生体的报道，但对细菌微生物知之甚少。在这项研究中，我们通过测序和分析从三个种群中获得的16S rRNA基因文库，检查了猪笼草中的细菌微生物，并鉴定了代表四个门（变形杆菌，硬毛，放线菌和拟杆菌）的总共18个操作分类单位（OTU）。保留在水稻品种TN1，Mudgo和ASD7上的N. lugens。几个OTU与先前报道的其他昆虫的次生共生体相似，包括木乃伊Glycapsis brimblecombei的内生共生体。在蚊子中的按蚊中发现，在螨虫西方螨中发现了Wolbachia。但是，检测到的OTU的种类和数量在N. lugens种群之间存在很大差异。此外，使用洋地黄毒苷标记的探针进行的原位杂交分析表明，OTU 1位于生物型1昆虫的卵子下卵巢组织和卵巢中，而OTU 2位于生物型2昆虫的卵子鞘前面。此外，在昆虫赖以为食的水稻植物组织的唾液鞘管中观察到大量细菌样生物。结果为褐飞虱所携带的细菌微生物的多样性以及特定的细菌微生物与发光猪笼草生物型之间的可能联系提供了指示。

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