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首页> 外文期刊>Applied Microbiology >Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes
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Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes

机译：通过基于焦磷酸测序的16S rRNA基因分析揭示了德国草原土壤的特定于地平线的细菌群落组成

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摘要

The diversity of bacteria in soil is enormous, and soil bacterial communities can vary greatly in structure. Here, we employed a pyrosequencing-based analysis of the V2-V3 16S rRNA gene region to characterize the overall and horizon-specific (A and B horizons) bacterial community compositions in nine grassland soils, which covered three different land use types. The entire data set comprised 752,838 sequences, 600,544 of which could be classified below the domain level. The average number of sequences per horizon was 41,824. The dominant taxonomic groups present in all samples and horizons were the Acidobacteria , Betaproteobacteria , Actinobacteria , Gammaproteobacteria , Alphaproteobacteria , Deltaproteobacteria , Chloroflexi , Firmicutes , and Bacteroidetes . Despite these overarching dominant taxa, the abundance, diversity, and composition of bacterial communities were horizon specific. In almost all cases, the estimated bacterial diversity ( H ′) was higher in the A horizons than in the corresponding B horizons. In addition, the H ′ was positively correlated with the organic carbon content, the total nitrogen content, and the C-to-N ratio, which decreased with soil depth. It appeared that lower land use intensity results in higher bacterial diversity. The majority of sequences affiliated with the Actinobacteria , Bacteroidetes , Cyanobacteria , Fibrobacteres , Firmicutes , Spirochaetes , Verrucomicrobia , Alphaproteobacteria , Betaproteobacteria , and Gammaproteobacteria were derived from A horizons, whereas the majority of the sequences related to Acidobacteria , Chloroflexi , Gemmatimonadetes , Nitrospira , TM7, and WS3 originated from B horizons. The distribution of some bacterial phylogenetic groups and subgroups in the different horizons correlated with soil properties such as organic carbon content, total nitrogen content, or microbial biomass.
机译：土壤中细菌的多样性是巨大的，土壤细菌群落的结构可以有很大差异。在这里，我们对V2-V3 16S rRNA基因区域进行了基于焦磷酸测序的分析，以表征涵盖三种不同土地利用类型的九种草原土壤的总体和特定水平（A和B水平）细菌群落组成。整个数据集包含752,838个序列，其中600,544个序列可以在域级别以下分类。每个视野的平均序列数为41,824。在所有样品和视野中出现的主要分类类别是嗜酸细菌，β变形细菌，放线菌，γ变形细菌，Alpha变形细菌，Delta变形细菌，绿叶弯曲菌，Firmicutes和拟杆菌。尽管有这些总体优势类群，细菌群落的丰度，多样性和组成是特定于地平线的。在几乎所有情况下，A层的估计细菌多样性（H'）高于相应的B层。另外，H'与有机碳含量，总氮含量和碳氮比呈正相关，随土壤深度的增加而降低。较低的土地利用强度似乎导致较高的细菌多样性。与放线杆菌，拟杆菌，蓝细菌，纤维杆菌，硬毛，螺旋藻，疣状微生物，丙型杆菌，β型细菌和丙型细菌有关的大多数序列均来自视界，而大多数与嗜酸菌，硝化杆菌，叶绿体，细菌，拟杆菌， ，并且WS3源自B层。不同视野中某些细菌系统发生族群和亚群的分布与土壤特性（例如有机碳含量，总氮含量或微生物生物量）相关。

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