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首页> 外文期刊>Applied Microbiology >Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F
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Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F

机译：F型肉毒杆菌神经毒素基因的序列多样性

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Botulism due to type F botulinum neurotoxin (BoNT/F) is rare (<1% of cases), and only a limited number of clostridial strains producing this toxin type have been isolated. As a result, analysis of the diversity of genes encoding BoNT/F has been challenging. In this study, the entire bont/F nucleotide sequences were determined from 33 type F botulinum toxin-producing clostridial strains isolated from environmental sources and botulism outbreak investigations. We examined proteolytic and nonproteolytic Clostridium botulinum type F strains, bivalent strains, including Bf and Af, and Clostridium baratii type F strains. Phylogenetic analysis revealed that the bont/F genes examined formed 7 subtypes (F1 to F7) and that the nucleotide sequence identities of these subtypes differed by up to 25%. The genes from proteolytic (group I) C. botulinum strains formed subtypes F1 through F5, while the genes from nonproteolytic (group II) C. botulinum strains formed subtype F6. Subtype F7 was composed exclusively of bont/F genes from C. baratii strains. The region of the bont/F5 gene encoding the neurotoxin light chain was found to be highly divergent compared to the other subtypes. Although the bont/F5 nucleotide sequences were found to be identical in strains harboring this gene, the gene located directly upstream ( ntnh/F ) demonstrated sequence variation among representative strains of this subtype. These results demonstrate that extensive nucleotide diversity exists among genes encoding type F neurotoxins from strains with different phylogenetic backgrounds and from various geographical sources.
机译：由F型肉毒杆菌神经毒素（BoNT / F）引起的肉毒中毒很少见（占病例的1％），并且仅分离出了有限数量的产生这种毒素类型的梭菌菌株。结果，对编码BoNT / F的基因的多样性进行分析具有挑战性。在这项研究中，完整的bont / F核苷酸序列是从33种产生F型肉毒杆菌毒素的梭菌菌株中确定的，这些菌株从环境来源和肉毒杆菌爆发调查中分离出来。我们检查了蛋白水解和非蛋白水解肉毒梭菌F型菌株，二价菌株，包括Bf和Af，以及梭状梭状芽胞杆菌F型菌株。系统发育分析表明，所检测的bont / F基因形成7个亚型（F1至F7），这些亚型的核苷酸序列同一性相差最多25％。来自蛋白水解（组I）的肉毒梭菌菌株的基因形成F1至F5亚型，而来自非蛋白水解（组II）的肉毒梭菌菌株的基因形成F6亚型。 F7亚型仅由C. baratii菌株的bont / F基因组成。与其他亚型相比，发现编码神经毒素轻链的bont / F5基因区域高度不同。尽管在具有该基因的菌株中发现了bont / F5核苷酸序列相同，但直接位于上游的基因（ntnh / F）在该亚型的代表性菌株中显示出序列变异。这些结果证明，在来自具有不同系统发育背景的菌株和来自不同地理来源的编码F型神经毒素的基因之间存在广泛的核苷酸多样性。

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