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首页> 外文期刊>Applied Microbiology >Modulation of Responses of Vibrio parahaemolyticus O3:K6 to pH and Temperature Stresses by Growth at Different Salt Concentrations
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Modulation of Responses of Vibrio parahaemolyticus O3:K6 to pH and Temperature Stresses by Growth at Different Salt Concentrations

机译：在不同盐浓度下副溶血性弧菌O3：K6对pH和温度胁迫响应的调节

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Vibrio parahaemolyticus inhabits marine, brackish, and estuarine waters worldwide, where fluctuations in salinity pose a constant challenge to the osmotic stress response of the organism. Vibrio parahaemolyticus is a moderate halophile, having an absolute requirement for salt for survival, and is capable of growth at 1 to 9% NaCl. It is the leading cause of seafood-related bacterial gastroenteritis in the United States and much of Asia. We determined whether growth in differing NaCl concentrations alters the susceptibility of V. parahaemolyticus O3:K6 to other environmental stresses. Vibrio parahaemolyticus was grown at a 1% or 3% NaCl concentration, and the growth and survival of the organism were examined under acid or temperature stress conditions. Growth of V. parahaemolyticus in 3% NaCl versus that in 1% NaCl increased survival under both inorganic (HCl) and organic (acetic acid) acid conditions. In addition, at 42°C and ?20°C, 1% NaCl had a detrimental effect on growth. The expression of lysine decarboxylase (encoded by cadA ), the organism's main acid stress response system, was induced by both NaCl and acid conditions. To begin to address the mechanism of regulation of the stress response, we constructed a knockout mutation in rpoS , which encodes the alternative stress sigma factor, and in toxRS , a two-component regulator common to many Vibrio species. Both mutant strains had significantly reduced survival under acid stress conditions. The effect of V. parahaemolyticus growth in 1% or 3% NaCl was examined using a cytotoxicity assay, and we found that V. parahaemolyticus grown in 1% NaCl was significantly more toxic than that grown in 3% NaCl.
机译：副溶血性弧菌在全世界的海水，微咸水和河口水域中栖居，那里的盐度波动不断地挑战着生物体的渗透胁迫反应。副溶血性弧菌是中等嗜盐菌，对盐具有绝对的生存要求，并且能够在1％至9％的NaCl中生长。它是在美国和亚洲大部分地区与海鲜有关的细菌性肠胃炎的主要原因。我们确定了不同NaCl浓度下的生长是否会改变副溶血弧菌O3：K6对其他环境胁迫的敏感性。副溶血性弧菌在1％或3％的NaCl浓度下生长，并在酸性或温度胁迫条件下检查了该生物的生长和存活。在无机（HCl）和有机（乙酸）酸条件下，溶血弧菌在3％NaCl中的生长比在1％NaCl中的生长增加了存活率。另外，在42℃和〜20℃，1％NaCl对生长有不利影响。 NaCl和酸性条件均可诱导生物体主要的酸应激反应系统赖氨酸脱羧酶（由cadA编码）的表达。为了开始解决压力响应调节的机制，我们在rpoS中构建了敲除突变，该突变编码替代性的应力sigma因子，在toxRS中构建了许多弧菌物种共有的两组分调节剂。两种突变菌株在酸性胁迫条件下的存活率均显着降低。使用细胞毒性测定法检查了溶血弧菌在1％或3％NaCl中的生长效果，我们发现溶血弧菌在1％NaCl中生长的毒性明显高于3％NaCl。

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