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首页> 外文期刊>Applied Microbiology >Common Ancestry and Novel Genetic Traits of Francisella novicida-Like Isolates from North America and Australia as Revealed by Comparative Genomic Analyses
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Common Ancestry and Novel Genetic Traits of Francisella novicida-Like Isolates from North America and Australia as Revealed by Comparative Genomic Analyses

机译：比较基因组分析揭示了来自北美和澳大利亚的新弗朗西斯菌样菌株的共同祖先和新的遗传特征

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Francisella novicida is a close relative of Francisella tularensis , the causative agent of tularemia. The genomes of F. novicida -like clinical isolates 3523 (Australian strain) and Fx1 (Texas strain) were sequenced and compared to F. novicida strain U112 and F. tularensis strain Schu S4. The strain 3523 chromosome is 1,945,310 bp and contains 1,854 protein-coding genes. The strain Fx1 chromosome is 1,913,619 bp and contains 1,819 protein-coding genes. NUCmer analyses revealed that the genomes of strains Fx1 and U112 are mostly colinear, whereas the genome of strain 3523 has gaps, translocations, and/or inversions compared to genomes of strains Fx1 and U112. Using the genome sequence data and comparative analyses with other members of the genus Francisella , several strain-specific genes that encode putative proteins involved in RTX toxin production, polysaccharide biosynthesis/modification, thiamine biosynthesis, glucuronate utilization, and polyamine biosynthesis were identified. The RTX toxin synthesis and secretion operon of strain 3523 contains four open reading frames (ORFs) and was named rtxCABD . Based on the alignment of conserved sequences upstream of operons involved in thiamine biosynthesis from various bacteria, a putative THI box was identified in strain 3523. The glucuronate catabolism loci of strains 3523 and Fx1 contain a cluster of nine ORFs oriented in the same direction that appear to constitute an operon. Strains U112 and Schu S4 appeared to have lost the loci for RTX toxin production, thiamine biosynthesis, and glucuronate utilization as a consequence of host adaptation and reductive evolution. In conclusion, comparative analyses provided insights into the common ancestry and novel genetic traits of these strains.
机译：新弗朗西斯菌（Francisella novicida）是tularemia的病原体Francisella tularensis的近亲。对新孢子虫样临床分离株3523（澳大利亚菌株）和Fx1（德克萨斯菌株）的基因组进行测序，并将其与新孢子虫菌株U112和土拉弗朗西斯菌菌株Schu S4进行比较。 3523染色体染色体为1,945,310 bp，包含1,854个蛋白质编码基因。菌株Fx1染色体为1,913,619 bp，包含1,819个蛋白质编码基因。 NUCmer分析显示，菌株Fx1和U112的基因组大部分是共线性的，而与菌株Fx1和U112的基因组相比，菌株3523的基因组具有缺口，易位和/或倒位。使用基因组序列数据并与弗朗西斯菌属的其他成员进行比较分析，鉴定了几个特定于菌株的基因，这些基因编码了参与RTX毒素产生，多糖生物合成/修饰，硫胺素生物合成，葡萄糖醛酸酯利用和多胺生物合成的推定蛋白质。菌株3523的RTX毒素合成和分泌操纵子包含四个开放阅读框（ORF），命名为rtxCABD。基于比对从各种细菌中硫胺素生物合成中涉及的操纵子上游保守序列的比对，在3523菌株中鉴定出一个推定的THI盒。3523和Fx1菌株的葡萄糖醛酸分解代谢基因座包含9个ORF簇，它们的方向相同构成操纵子。由于宿主适应和还原性进化，U112和Schu S4菌株似乎失去了RTX毒素产生，硫胺素生物合成和葡萄糖醛酸酯利用的基因座。总之，比较分析为这些菌株的共同血统和新的遗传特征提供了见识。

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