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Evidence for Direct Electron Transfer by a Gram-Positive Bacterium Isolated from a Microbial Fuel Cell

机译：从微生物燃料电池分离出的革兰氏阳性细菌进行直接电子转移的证据

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Despite their importance in iron redox cycles and bioenergy production, the underlying physiological, genetic, and biochemical mechanisms of extracellular electron transfer by Gram-positive bacteria remain insufficiently understood. In this work, we investigated respiration by Thermincola potens strain JR, a Gram-positive isolate obtained from the anode surface of a microbial fuel cell, using insoluble electron acceptors. We found no evidence that soluble redox-active components were secreted into the surrounding medium on the basis of physiological experiments and cyclic voltammetry measurements. Confocal microscopy revealed highly stratified biofilms in which cells contacting the electrode surface were disproportionately viable relative to the rest of the biofilm. Furthermore, there was no correlation between biofilm thickness and power production, suggesting that cells in contact with the electrode were primarily responsible for current generation. These data, along with cryo-electron microscopy experiments, support contact-dependent electron transfer by T. potens strain JR from the cell membrane across the 37-nm cell envelope to the cell surface. Furthermore, we present physiological and genomic evidence that c -type cytochromes play a role in charge transfer across the Gram-positive bacterial cell envelope during metal reduction.
机译：尽管它们在铁氧化还原循环和生物能产生中具有重要意义，但对革兰氏阳性细菌转移细胞外电子的潜在生理，遗传和生化机制仍知之甚少。在这项工作中，我们研究了Thermincola potens菌株JR的呼吸作用，Jr是使用不溶性电子受体从微生物燃料电池阳极表面获得的革兰氏阳性分离株。根据生理实验和循环伏安法测量，我们没有发现可溶的氧化还原活性成分分泌到周围介质中的证据。共聚焦显微镜检查显示出高度分层的生物膜，其中与电极表面接触的细胞相对于其余生物膜成比例地存活。此外，生物膜厚度与发电量之间没有相关性，表明与电极接触的细胞主要负责电流的产生。这些数据与低温电子显微镜实验一起，支持强力螺旋杆菌菌株JR从细胞膜跨37 nm细胞膜到细胞表面的接触依赖性电子转移。此外，我们提出了生理和基因组学证据，即在金属还原过程中c型细胞色素在跨革兰氏阳性细菌细胞包膜的电荷转移中发挥了作用。

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