Expression Vectors for Acinetobacter baylyi ADP1

Charles Daniel Murin; Kristy Segal; Anton Bryksin; Ichiro Matsumura

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Expression Vectors for Acinetobacter baylyi ADP1

机译：巴氏不动杆菌ADP1的表达载体

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Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.

机译：拜氏不动杆菌ADP1具有天然能力，并且擅长同源重组，因此无需限制酶切或连接反应即可转化。然而，该系统的表达载体尚未广泛获得。在这里，我们描述了可诱导表达载体的构建和表征，该载体可作为质粒在拟南芥中复制或整合入其染色体的非必需部分。这些工具将促进这种有前途的模型生物中基因和基因组的工程改造。

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《Applied Microbiology》 |2012年第1期|共4页
作者
Charles Daniel Murin; Kristy Segal; Anton Bryksin; Ichiro Matsumura;
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1. Expression Vectors for Acinetobacter baylyi ADP1 [J] . Charles Daniel Murin, Kristy Segal, Anton Bryksin, Applied and Environmental Microbiology . 2012,第1期

机译：巴氏不动杆菌ADP1的表达载体
2. Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1 [J] . Helge Jans Jan?en, Alexander Steinbüchel Applied Microbiology and Biotechnology . 2014,第4期

机译：通过缺失二酰基甘油激酶基因和异源过表达Bayfly Alytobacter ADP1的atfA来生产大肠杆菌中的三酰基甘油
3. Quantitative measurement of pH influence on SalR regulated gene expression in Acinetobacter baylyi ADP1 [J] . Li Chao, Wang Hui, Zhou Lin, Journal of Microbiological Methods . 2009,第1期

机译：pH值对Bayly不动杆菌ADP1中SalR调控基因表达的影响的定量测量
4. Identification of an unexpected de novo metabolite from Acinetobacter baylyi ADP1: a particular challenge for the HRMS and HR/MSn arsenal [C] . Lucille Stuani, Christophe Lechaplais, Ekaterina Darii, International Mass Spectrometry Conference . 2014

机译：鉴定来自Acinetobacters ADP1的意外de Novo代谢物：HRMS和HR / MSN阿森纳的特殊挑战
5. Genetic Manipulation of Acinetobacter baylyi ADP1 to Enhance Biofuel Precursor Production. [D] . Ukey, Rahul. 2016

机译：遗传性不动杆菌ADP1的基因操纵，以提高生物燃料前体的生产。
6. Expression Vectors for Acinetobacter baylyi ADP1 [O] . Charles Daniel Murin, Kristy Segal, Anton Bryksin, 2012

机译：巴氏不动杆菌ADP1的表达载体
7. Expression Vectors for Acinetobacter baylyi ADP1 [O] . Murin, Charles Daniel, Segal, Kristy, Bryksin, Anton, 2011

机译：巴氏不动杆菌ADP1的表达载体
8. Automatic Code Vectorization of Arithmetic Expressions by Bottom-Up Structure Recognition [R] . Goldman, V. V., Vanhulzen, J. A. 1988

机译：自下而上结构识别的算术表达式自动编码矢量化

Expression Vectors for Acinetobacter baylyi ADP1

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