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首页> 外文期刊>Applied Microbiology >Molecular Survey and Genetic Identification of Anaplasma Species in Goats from Central and Southern China
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Molecular Survey and Genetic Identification of Anaplasma Species in Goats from Central and Southern China

机译：中国中部和南部山羊无性体的分子调查和遗传鉴定

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Anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. Few studies have been carried out on Anaplasma infections in central and southern China. This study was conducted to determine the coinfection rates of Anaplasma ovis , A. bovis , and A. phagocytophilum from 262 field blood samples of goats in these regions. The average prevalences of single infection of A. ovis , A. bovis , and A. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. Coinfection of A. ovis and A. bovis was dominant, with an infection rate of 27.1%. Coinfection of A. ovis and A. phagocytophilum was 1.9% and that of A. bovis and A. phagocytophilum was 4.2%. Three-pathogen coinfection was found in three of four investigated provinces with a prevalence between 0 and 5.3%. The accuracy of the PCR results was corroborated by sequencing. Analysis of the 16S rRNA gene sequences of A. bovis and A. phagocytophilum confirmed the presence of these pathogens at the investigated sites and indicated the possible genetic diversity of A. phagocytophilum . Field blood inoculation of experimental animals led to successful identification and observation of the morphological shapes of A. bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites.
机译：浆膜物种是专性的细胞内立克次氏病病原体，会影响人类和动物的健康。在中国中部和南部，很少有关于无性体感染的研究。进行了这项研究，以确定来自这些地区的262个山羊野外血液样本中的牛无形体，牛毛线虫和吞噬性线虫的合并感染率。单次感染A. ovis，A。bovis和A. phagocytophilum的平均患病率分别为15.3％，16.0％和6.1％。牛肝菌和牛肝菌的合并感染占主导地位，感染率为27.1％。猪曲霉和吞噬曲霉的共同感染率为1.9％，牛曲霉和吞噬菌的共同感染为4.2％。在四个调查的省份中有三个发现了三病原体合并感染，患病率为0％至5.3％。通过测序证实了PCR结果的准确性。分析牛链球菌和嗜食曲霉的16S rRNA基因序列证实这些病原体存在于研究的地点，并指出了嗜食曲霉的遗传多样性。实验动物的野外血液接种成功地鉴定和观察了绵羊被感染的单核细胞中牛曲霉的形态。用osp。avis msp4序列进行的系统发育研究表明，北部绵羊的A. ovis基因型与研究地点的山羊的基因型不同。

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